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Título

Expression profiling of the sex-related gene Dmrt1 in adults of the lusitanian toadfish halobatrachus didactylus (bloch and schneider, 1801)

Autor Úbeda-Manzanaro, María ; Merlo, Manuel A. ; Ortiz-Delgado, Juan B. ; Rebordinos, Laureana; Sarasquete, Carmen
Palabras clave Dmrt1
Expression pattern
Spermatogenesis
In situ hybridization
Oogenesis
Immunochemistry
Fecha de publicación 10-feb-2014
EditorElsevier
Citación Gene 535(2): 255-265 (2014)
ResumenDoublesex and mab-3 related transcription factor 1 (Dmrt1) gene is a widely conserved gene involved in sex determination and differentiation across phyla. To gain insights on Dmrt1 implication for fish gonad cell differentiation and gametogenesis development, its mRNA was isolated from testis and ovary from the Lusitanian toadfish (Halobatrachus didactylus). The cDNA from Dmrt1 was synthesized and cloned, whereas its quantitative and qualitative gene expression, as well as its protein immunolocalization, were analyzed. A main product of 1.38. kb, which encodes a protein of 295 aa, was reported, but other minority Dmrt1 products were also identified by RACE-PCR. This gene is predominantly expressed in testis (about 20 times more than in other organs or tissues), specially in spermatogonia, spermatocytes and spermatids, as well as in somatic Sertoli cells, indicating that Dmrt1 plays an important role in spermatogenesis. Although Dmrt1 transcripts also seem to be involved in oogenesis development, and it cannot be excluded that toadfish Dmrt1 could be functionally involved in other processes not related to sex. © 2013 Elsevier B.V.
URI http://hdl.handle.net/10261/102425
DOI10.1016/j.gene.2013.11.021
Identificadoresdoi: 10.1016/j.gene.2013.11.021
issn: 0378-1119
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