English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10226
Título

Matrix effect in liquid chromatography–electrospray ionization mass spectrometry analysis of benzoxazinoid derivatives in plant material.

AutorVillagrasa, Marta; Guillamón, Miriam; Eljarrat, Ethel; Barceló, Damià
Palabras claveAllelochemicals
Benzoxazinoid derivatives
Benzoxazinones
Benzoxazolinones
Electrospray ionization
Ion suppression
LC–MS
Matrix effect
Wheat
Fecha de publicación21-abr-2007
EditorElsevier
CitaciónJournal of Chromatography A 1157(1-2): 108-114 (2007)
ResumenDespite the increasing success of liquid chromatography (LC) coupled to mass spectrometry (MS), matrix effects have limited the ESI applications. Matrix effects result from co-eluting residual matrix components affecting the ionization efficiency of target analytes and can lead to erroneous results. The present work evaluates the LC–electrospray ionization (ESI)-MS behaviour of benzoxazinoid derivatives in wheat plant material as a case where endogenous matrix components were found to interfere with the ionization of target analytes. When LC–MS was applied to the analysis of foliage and root extracts, significant signal suppressions were observed. To compensate for this matrix signal suppression, different methods were applied: the use of internal standards, the application of standard dilution method and the dilution of the extracts before instrumental determination. The results obtained indicated that evaluation of matrix effects should become an integrated part of quantitative LC–ESI-MS method development and validation.
Descripción7 pages, 5 figures.-- Printed version published on July 20, 2007.
Versión del editorhttp://dx.doi.org/10.1016/j.chroma.2007.04.040
URIhttp://hdl.handle.net/10261/10226
DOI10.1016/j.chroma.2007.04.040
ISSN0021-9673
Aparece en las colecciones: (IDAEA) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.