Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/102215
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Dietary protein quality differentially regulates trypsin enzymes at the secretion and transcription level in Panulirus argus by distinct signaling pathways |
Autor: | Perera, Erick CSIC ORCID ; Rodríguez-Viera, Leandro; Rodríguez-Casariego, Javier; Fraga, Iliana; Carrillo, Olimpia; Martínez-Rodríguez, Gonzalo CSIC ORCID ; Mancera, Juan Miguel | Palabras clave: | Spiny lobster Protein digestion Digest regulation Trypsin Panulirus argus |
Fecha de publicación: | 1-mar-2012 | Editor: | Company of Biologists | Citación: | Journal of Experimental Biology 215: 853-862 (2012) | Resumen: | The effects of pelleted diets with different protein composition (fish, squid or soybean meals as main protein sources) on trypsin secretion and expression were studied in the lobster Panulirus argus. Trypsin secretion was shown to be maximal 4h after ingestion. At this time, fish-and squid-based diets induced trypsin secretion, as well as up-regulation of the major trypsin isoform at the transcription level. While fish-and squid-based diets elicited a prandial response, soybean-based diet failed to stimulate the digestive gland to secrete trypsin into the gastric fluid or induce trypsin expression above the levels observed in fasting lobsters. In vitro assays showed that intact proteins rather than protein hydrolysates stimulate trypsin secretion in the lobster. However, the signal for trypsin transcription appears to be different to that for secretion and is probably mediated by the appearance of free amino acids in the digestive gland, suggesting a stepwise regulation of trypsin enzymes during digestion. We conclude that trypsin enzymes in P. argus are regulated at the transcription and secretion level by the quality of dietary proteins through two distinct signaling pathways. Our results indicate that protein digestion efficiency in spiny lobsters can be improved by selecting appropriated protein sources. However, other factors like the poor solubility of dietary proteins in dry diets could hamper further enhancement of digestion efficiency. © 2012. Published by The Company of Biologists Ltd. | URI: | http://hdl.handle.net/10261/102215 | DOI: | 10.1242/jeb.063925 | Identificadores: | doi: 10.1242/jeb.063925 issn: 0022-0949 e-issn: 1477-9145 |
Aparece en las colecciones: | (ICMAN) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
28
checked on 22-abr-2024
WEB OF SCIENCETM
Citations
25
checked on 23-feb-2024
Page view(s)
302
checked on 23-abr-2024
Download(s)
91
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.