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dc.contributor.authorSantos, Fernando-
dc.contributor.authorMeyerdierks, Anke-
dc.contributor.authorPeña, Arantxa-
dc.contributor.authorRosselló-Mora, Ramón-
dc.contributor.authorAmann, Rudolf-
dc.contributor.authorAntón, Josefa-
dc.identifierdoi: 10.1111/j.1462-2920.2007.01289.x-
dc.identifierissn: 1462-2912-
dc.identifier.citationEnvironmental Microbiology 9(7): 1711-1723 (2007)-
dc.description.abstractHypersaline environments, such as crystallizer ponds of solar salterns, show one of the highest concentration of viruses reported for aquatic systems. All the halophages characterized so far are isolates obtained by cultivation from described haloarchaeal species that have only low abundance in the environment. We employed a culture-independent metagenomic approach to analyse for the first time complete genomes in the halophage community and explored the in situ diversity by transmission electron microscopy and pulsed-field gel electrophoresis. We report the genomic sequence of a not yet isolated halophage (named as environmental halophage 1 'EHP-1′) whose DNA was obtained from crystallizer samples with a salinity of 31%. The sequenced genome has a size of 35 kb and a G + C content around 51%. The G + C content is lower than that of previously characterized halophages. However, G + C content and codon usage in EHP-1 are similar to the recently cultivated and sequenced Haloquadratum walsbyi, the major prokaryotic component in solar salterns around the world. Forty open reading frames have been predicted, including genes that putatively code for proteins involved in DNA replication (ribonucleotide reductases, thymidylate kinase) normally found in lytic viruses. © 2007 The Authors.-
dc.description.sponsorshipThis work was funded by the FP6-EU Network of Excellence Marine Genomics (Contract Nb 505403), the Max Planck Society, the Spanish Ministry of Science and Education Grants BOS2003-05198-C02-01 and -02 and the Generalitat Valenciana (Grant ACOMP06/097)-
dc.publisherBlackwell Publishing-
dc.titleMetagenomic approach to the study of halophages: The environmental halophage 1-
dc.description.versionPeer Reviewed-
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