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Title

Detection of SJNNV and RGNNV genotypes using a relative quantification RT-PCR assay

AuthorsCherif, N.; López-Jimena, B.; García Raso, J. Enrique; Cano, I. CSIC; Castro, D.; Borrego, Juan J.; Alonso, M. C.; Hammami, S.
Issue DateJun-2011
PublisherWiley-Blackwell
CitationJournal of Applied Ichthyology 27(3): 805-812 (2011)
AbstractViral Encephalopathy and Retinopathy (VER), is caused by a nodavirus included within the Betanodavirus genus of the Nodaviridae family. This disease affects more than 30 marine fish species worldwide and has been a major obstacle in the aquaculture industry; control of the disease is based on virus detection, essentially in carrier specimens. This study describes a real time PCR procedure for viral nervous necrosis virus detection from several organs of sea bass, Senegalese sole, and gilt-head sea bream, from fish displaying either clinical symptoms or asymptomatic cases. The sensitivity of this technique was about 106-fold higher than that of the conventional RT-PCR. The newly designed primers detected nodavirus isolates belonging to the RGNNV and SJNNV genotypes. © 2010 Blackwell Verlag, Berlin.
URIhttp://hdl.handle.net/10261/101929
DOI10.1111/j.1439-0426.2010.01592.x
Identifiersdoi: 10.1111/j.1439-0426.2010.01592.x
issn: 0175-8659
e-issn: 1439-0426
Appears in Collections:(ICMAN) Artículos

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