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dc.contributor.authorGodoy, César A.-
dc.contributor.authorRivas, Blanca de las-
dc.contributor.authorGuisán, José Manuel-
dc.identifierdoi: 10.1016/j.procbio.2014.04.020-
dc.identifierissn: 1359-5113-
dc.identifier.citationProcess Biochemistry 49: 1324- 1331 (2014)-
dc.description.abstractImmobilized enzymes are preferred over their soluble counterparts due to their robustness in harsh industrial processes; the most stable enzyme derivatives are often produced through multipoint covalent attachment (MCA). However, most enzymes are unable to establish optimal MCA to electrophile-type supports given the heterogeneous distribution and/or low content of primary amino groups on their surfaces; this restricts both the diversity of areas prone to react and the number of attachments to the support. To overcome this we propose combining site-directed immobilization and protein engineering to increase the number of bonds between a specific enzyme surface and a tailor-made support. We applied this novel strategy to engineered mutants of the lipase 2 from Geobacillus thermocatenulatus with one Cys exposed residue, that after genetic amination and/or chemical amination, were immobilized on glyoxyl-disulfide support using a site-directed MCA protocol. Two highly stabilized derivatives of chemically aminated lipase variants, in which site-directed MCA implied the surrounding surface of residues Cys344 or Cys40, were produced: the first one was 2.4-fold more productive than the reference derivative (648 g of hydrolyzed ester); the second derivative was 40% more selective (EPA/DHA molar ratio) and as active (1 μmol g catalyst-1 min-1) as the reference in the production of PUFAs. © 2014 Elsevier Ltd.-
dc.description.sponsorshipCICYT (project BIO-2005-6018576), Madrid Region council CAM (grants S0505/PPQ/0344) and CSIC-Spain.-
dc.subjectTailor-made support-
dc.subjectLipase mutants-
dc.subjectSite-directed immobilization-
dc.subjectCovalent immobilization-
dc.titleSite-directing an intense multipoint covalent attachment (MCA) of mutants of the Geobacillus thermocatenulatus lipase 2 (BTL2): Genetic and chemical amination plus immobilization on a tailor-made support-
dc.description.versionPeer Reviewed-
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