English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10156
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Identification of commercial hake and grenadier species by proteomic analysis of the parvalbumin fraction

AutorCarrera, Mónica ; Cañas, Benito; Piñeiro, Carmen ; Vázquez, Jesús ; Gallardo, José Manuel
Palabras claveFish identification
MALDI-TOF MS
Merlucciidae
Parvalbumin
Fecha de publicación23-ago-2006
EditorJohn Wiley & Sons
CitaciónProteomics 6(19): 5278-5287 (2006)
ResumenAnalysis of parvalbumin fractions through proteomic methodologies allowed the differential classification of ten commercial, closely related species of the family Merlucciidae. Muscle extracts from nine hake species of the genus Merluccius including two subspecies of Merluccius australis (australis and polylepsis) and one grenadier species Macruronus novaezelandiae with two populations (novaezelandiae and magellanicus) were evaluated by 2-DE and MALDI-TOF MS. 2-DE demonstrated that the species tested displayed a low intra-specific degree of polymorphism and the isoform patterns were noticeably species-specific. MALDI-TOF mass fingerprints showed clear differences in the pattern of peptides produced by tryptic digestion between the Merluccius and the Macruronus, making the genus differentiation possible. In addition, a selective peptide mass present in the spectra from certain hakes allowed its classification in two groups: Euro-African and American hakes. Besides, some specific masses allowed a clearly individual identification for M. bilinearis, M. australis polylepsis, M. australis australis, M. productus, M. paradoxus and M. polli, while the rest of the hake species can be grouped in two clusters, comprising M. hubbsi and M. gayi in one and M. merluccius and M. capensis in the other.
Descripción10 pages, 3 figures.-- PMID: 16927426 [PubMed].-- Supplementary information (Results and discussion, figure S1, tables S1-S5, 8 pages) available at: http://www.wiley-vch.de/contents/jc_2120/2006/pro200500899_s.pdf
Printed version published Oct 2006.
Versión del editorhttp://dx.doi.org/10.1002/pmic.200500899
URIhttp://hdl.handle.net/10261/10156
DOI10.1002/pmic.200500899
ISSN1615-9853
Aparece en las colecciones: (IIM) Artículos
(CBM) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.