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dc.contributor.authorCarrera, Mónica-
dc.contributor.authorCañas, Benito-
dc.contributor.authorPiñeiro, Carmen-
dc.contributor.authorVázquez, Jesús-
dc.contributor.authorGallardo, José Manuel-
dc.identifier.citationJournal of Proteome Research 6(8): 3070-3080 (2007)en_US
dc.description11 pages, 7 figures.-- PMID: 17622168 [PubMed].-- Supplementary information (Results and discussion, figure S1, table S1, 2 pages) available at: http://pubs.acs.org/doi/suppl/10.1021/pr0701963/suppl_file/pr0701963si20070521_060524.pdf.en_US
dc.descriptionPrinted version published on Aug 3, 2007.-
dc.description.abstractThe characterization by de novo peptide sequencing of the different protein nucleoside diphosphate kinase B (NDK B) from all the commercial hakes and grenadiers belonging to the family Merlucciidae is reported. A classical proteomics approach, consisting of two-dimmensional gel electrophoresis, tryptic in-gel digestion of the excised spots, MALDI-TOF MS, LC−MS/MS, and nanoESI−MS/MS analyses, was followed for the purification and characterization of the different isoforms of the NDK B. Fragmentation spectra were used for de novo peptide sequence. A high degree of homology was found between the sequences of all the species studied and the NDK B sequence from Gillichthys mirabilis, which is accessible in the protein databases. Particular attention was paid to the differential characterization of species-specific peptides that could be used for fish authentication purposes. These findings allowed us to propose a rapid and effective classification method, based in the detection of these biomarker peptides using the selective ion reaction monitoring (SIRM) scan mode in mass spectrometry.en_US
dc.description.sponsorshipWe acknowledge Freiremar S.A. and Cetmar for their assistance in the collection of hakes and grenadiers used in this study. This work was supported by the Comisión Interministerial de Ciencia y Tecnología (CICyT) (Project AGL2000-0440-P4-02). B.C. is supported by the Ramón y Cajal program (RYC-2003) under the auspices of the Spanish Ministry of Science and Technology.en_US
dc.format.extent147288 bytes-
dc.publisherAmerican Chemical Societyen_US
dc.subjectNucleoside diphosphate Kinaseen_US
dc.subjectDe novo sequencingen_US
dc.subjectMass spectrometryen_US
dc.subjectSelective ion reaction monitoringen_US
dc.titleDe novo mass spectrometry sequencing and characterization of species-specific peptides from nucleoside diphosphate kinase B for the classification of commercial fish species belonging to the family Merlucciidaeen_US
dc.description.peerreviewedPeer revieweden_US
dc.contributor.funderComisión Interministerial de Ciencia y Tecnología, CICYT (España)-
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)-
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