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Title

A study on the heterogeneity of the light-harvesting complex II from Ectothiorhodospira sp. after acid/chaotropic treatment

AuthorsBuche, André ; Picorel Castaño, Rafael
KeywordsLight-Harvesting Protein Complexes
Photosynthetic Reaction Center Complex Proteins
Bacteriochlorophylls
Issue Date2000
PublisherZeitschrift für Naturforschung
CitationZeitschrift für Naturforschung - Section C Journal of Biosciences 55 (11-12): 943-947 (2000)
AbstractThe light-harvesting complex II of the purple bacteria has two strong near infrared electronic absorption bands, around 800 (B800) and 850 (B850) nm, arising from the Qy transitions of bacteriochlorophyll a. It was previously reported that under some specific acid/chaotropic conditions the B850 bacteriochlorophyll of the light-harvesting complex II of Ectothiorhodospira sp. are strongly reorganized. Part of these pigments absorbs at 843 nm while another set absorbs around 858 nm. The current work should investigate whether a mix of two different complexes could generate the 843- and 858-nm bands. Acid/chaotropic conditions inducing the reorganization of B850 were reproduced on a sample bound to an ionic-exchange column. The chromatographic pattern was found strongly homogeneous. The findings indicate that the heterogeneity of the reorganized B850 results from two forms of differently structured bacteriochlorophylls bound to the same polypeptide backbone.
Description5 Págs., 4 Figs. This work has been digitalized and published in 2013 by Verlag Zeitschrift für Naturforschung in cooperation with the Max Planck Society for the Advancement of Science under a Creative Commons Attribution-NoDerivs 3.0 Germany License, which permits to copy and redistribute the material in any medium or format.
URIhttp://hdl.handle.net/10261/101506
DOI10.1515/znc-2000-11-1215
ISSN0939-5075
Appears in Collections:(EEAD) Artículos
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