English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/101435
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 2 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Cloning and characterization of Vasa gene expression pattern in adults of the Lusitanian toadfish Halobatrachus didactylus

Autor Úbeda-Manzanaro, María ; Rebordinos, Laureana; Sarasquete, Carmen
Palabras clave Vasa
Toadfish
Spermatogenesis
Oogenesis
In situ hybridization
Immunochemistry
Expression pattern
Fecha de publicación jun-2014
EditorInter Research
Citación Aquatic Biology 21: 37-46 (2014)
ResumenThe Vasa gene is essential for germ cell development in eukaryotes. It encodes a RNA helicase, a member of the DEAD box protein family. Using the RACE method, we cloned the Vasa cDNA of the Lusitanian toadfish Halobatrachus didactylus, and analyzed quantitative and qualitative Vasa expression and its protein immunolocalization. We reported a main product of about 2.4 kb which encodes a protein of 615 amino acids, but other minority Vasa products were also identified by RACE-PCR. This gene is predominantly expressed in the ovaries and testes, although some relatively low extragonadal expression levels have also been identified. In situ hybridization and immunolocalization analysis during gametogenesis in the testes showed that toadfish Vasa mRNA was detected in spermatogonia, spermatocytes and spermatids. However, in the ovaries, Vasa mRNA was detected in early vitellogenic oocytes and in more advanced vitel-logenic stages, showing a very weak signal in oogonia, whereas the Vasa protein was evidenced in the cytoplasm of oogonia and previtellogenic oocytes, becoming weaker as the vitellogenic and maturation processes progress. These results suggest that toadfish Vasa homologues can play an important role in gametogenesis and germ cell development, but it could also be functionally implicated in other processes that are not as well known. © The authors 2014.
Versión del editorhttp://dx.doi.org/10.3354/ab00565
URI http://hdl.handle.net/10261/101435
DOI10.3354/ab00565
Identificadoresdoi: 10.3354/ab00565
issn: 1864-7782
e-issn: 1864-7790
Aparece en las colecciones: (ICMAN) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
vasa_gene_Ubeda.pdf2,94 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.