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Title

Isolation of prebiotic carbohydrates by supercritical fluid extraction. Scaling-up and economical feasibility

AuthorsMontañés, Fernando ; Fornari, Tiziana ; Olano, Agustín ; Ibáñez, Elena
KeywordsScale-up
Supercritical fractionation
Carbohydrate
Prebiotic
Economical study
Issue Date2012
PublisherElsevier
CitationJournal of Chromatography A 1250: 92-98 (2012)
AbstractProduction of prebiotic carbohydrates at competitive prices is a challenge nowadays since the well-established production processes involve many purification steps which are labour intensive and require important amounts of reagents and products thus increasing prebiotic's price. Several processes have been studied in our laboratory involving the use of Supercritical Fluid Technology to fractionate and purify carbohydrate solid mixtures. Research carried out at laboratory scale using theoretical mixtures (lactose/lactulose and galactose/tagatose), commercially available carbohydrate mixtures and carbohydrate mixtures produced by enzymatic transglycosylation and isomerized with complexating reagents demonstrated that purification of prebiotic carbohydrates was technically possible by supercritical fluid extraction. In the present work, the process optimized at laboratory scale to fractionate carbohydrate mixtures produced by enzymatic transglycosylation has been scaled-up to an industrial level and its economic feasibility has been simulated employing AspenONE ® V7.3 software to obtain consistent data supporting the interest of a potential investment for prebiotics production at large scale using supercritical fluids. © 2012 Elsevier B.V.
URIhttp://hdl.handle.net/10261/101366
DOIhttp://dx.doi.org/10.1016/j.chroma.2012.04.039
Identifiersdoi: 10.1016/j.chroma.2012.04.039
issn: 0021-9673
e-issn: 1873-3778
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