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Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion

AutorMartos, Gustavo ; Pineda-Vadillo, Carlos ; Miralles, Beatriz ; Alonso-Lebrero, Elena; López-Fandiño, Rosina ; Molina, Elena ; Belloque, Josefina
Palabras claveEgg white
Allergen
Digestion, IgE binding
Riboflavin binding protein
Fecha de publicación2012
EditorAmerican Chemical Society
CitaciónJournal of Agricultural and Food Chemistry 60(20): 5215-5220 (2012)
ResumenRiboflavin binding protein (RfBP) is a minor protein in hen egg; its potential involvement in egg allergy has seldom been studied. The aim of this work was to investigate the IgE binding capacity of RfBP before and after simulated gastrointestinal digestion. It was shown that digestion of RfBP mainly occurred during the gastric phase. The protein fragments resulting from the subsequent duodenal phase remained linked through disulfide bonds. Both the intact protein and its digests were subjected to inhibition ELISA with sera obtained from patients allergic to egg. The results revealed significant IgE binding to intact RfBP, whereas the digests showed reduced but substantial IgE binding levels, with serum-to-serum variability. The RfBP digests were then subjected to immunoblot with allergic patients' sera, and the IgE-reactive peptides were further analyzed by MALDI-TOF/TOF mass spectrometry for sequence determination. The RfBP sequence 41-84 was identified as a novel IgE binding peptide in patients allergic to egg. © 2012 American Chemical Society.
URIhttp://hdl.handle.net/10261/101341
DOI10.1021/jf3001586
Identificadoresdoi: 10.1021/jf3001586
issn: 0021-8561
e-issn: 1520-5118
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