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Hydrolyzed caseinomacropeptide conjugated galactooligosaccharides support the growth and enhance the bile tolerance in lactobacillus strains

AuthorsMuthaiyan, A.; Hernández-Hernández, Oswaldo ; Moreno, F. Javier ; Sanz, M. Luz ; Ricke, S. C.
Issue Date2012
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 60(27): 6839-6845 (2012)
AbstractIn this study bioactive caseinomacropeptide was conjugated with prebiotic galactooligosaccharides (hCMP:GOS) by Maillard reaction to synthesize value added prebiotic compounds to Lactobacillus strains. Growth study showed the ability of hCMP:GOS to serve as a sole carbon source for Lactobacillus strains. A significant amount of acetate and lactate was detected in cell free culture supernatant by HPLC. It demonstrated the ability of Lactobacillus strains to ferment the hCMP:GOS as a carbon source. In addition, hCMP:GOS grown Lactobacillus cells exhibited enhanced bile tolerance and retained 90% viability. Overall results of this study indicate that the hCMP conjugated GOS can be potential multipurpose prebiotic substrates to enhance the growth and bile tolerance in Lactobacillus strains and serve as a fermentable substrate to produce beneficial metabolites in the host. © 2012 American Chemical Society.
Identifiersdoi: 10.1021/jf301392y
issn: 0021-8561
e-issn: 1520-5118
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