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dc.contributor.authorPadilla, Beatriz-
dc.contributor.authorRuiz-Matute, Ana I.-
dc.contributor.authorBelloch, Carmela-
dc.contributor.authorCardelle-Cobas, Alejandra-
dc.contributor.authorCorzo, Nieves-
dc.contributor.authorManzanares, Paloma-
dc.date.accessioned2014-08-27T12:30:02Z-
dc.date.available2014-08-27T12:30:02Z-
dc.date.issued2012-
dc.identifierdoi: 10.1021/jf300852s-
dc.identifierissn: 0021-8561-
dc.identifiere-issn: 1520-5118-
dc.identifier.citationJournal of Agricultural and Food Chemistry 60(20): 5134-5141 (2012)-
dc.identifier.urihttp://hdl.handle.net/10261/101301-
dc.description.abstractThe β-galactosidase activity of 15 Kluyveromyces strains isolated from cheese belonging to Kluyveromyces lactis and Kluyveromyces marxianus species was tested for the production of oligosaccharides derived from lactose (GOS) and lactulose (OsLu). All Kluyveromyces crude cell extracts (CEEs) produced GOS, such as 6-galactobiose and 3′-, 4′-, and 6′-galactosyl- lactose. At 4 h of reaction, the main trisaccharide formed was 6′-galactosyl-lactose (20 g/100 g of total carbohydrates). The formation of OsLu was also observed by all CEEs tested, with 6-galactobiose, 6′-galactosyl-lactulose, and 1-galactosyl-lactulose being found in all of the reaction mixtures. The synthesis of trisaccharides predominated over other oligosaccharides. K. marxianus strain O3 produced the highest yields of GOS and OsLu after 4 h of reaction, reaching 42 g/100 g of total carbohydrates (corresponding to 80% lactose hydrolysis) and 45 g/100 g of total carbohydrates (corresponding to 87% lactulose hydrolysis), respectively. Therefore, the present study contributes to a better insight into dairy Kluyveromyces β-galactosidases and shows the feasibility of these enzymes to transglycosylate lactose and lactulose, producing high yields of prebiotic oligosaccharides. © 2012 American Chemical Society.-
dc.description.sponsorshipThis work was supported by Grants Consolider Ingenio 2010, Fun-C-Food, CSD2007-00063, INIA RM2007-00010, and the R + D program of the Comunidad de Madrid, Project ALIBIRD 2009/AGR-1469. Beatriz Padilla and Ana I. Ruiz-Matute thank their JAE Predoc and JAE Doc contracts from CSIC, respectively.-
dc.publisherAmerican Chemical Society-
dc.relationS2009/AGR-1469/ALIBIRD-
dc.rightsclosedAccess-
dc.subjectTransgalactosylation-
dc.subjectKluyveromyces lactis-
dc.subjectOsLu-
dc.subjectGOS-
dc.subjectKluyveromyces marxianus-
dc.titleEvaluation of oligosaccharide synthesis from lactose and lactulose using β-galactosidases from kluyveromyces isolated from artisanal cheeses-
dc.typeartículo-
dc.identifier.doi10.1021/jf300852s-
dc.date.updated2014-08-27T12:30:02Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderComunidad de Madrid-
dc.contributor.funderEuropean Commission-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
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