English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/101299
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Effects on intestinal microbiota of probiotic fermented milk used for prevention of antibiotic-associated diarrhoea

AuthorsTabasco, Raquel; Fontecha, F. Javier ; Peláez, Carmen ; Requena, Teresa
KeywordsAntibiotic-associated diarrhoea
Bifidobacterium lactis
Issue Date2012
CitationEuropean Food Research and Technology 235(6): 1199-1206 (2012)
AbstractProbiotic administration could prevent antibiotic-associated diarrhoea due to the restoration of an intestinal microbiota imbalance caused by antibiotics. The effect of the probiotics Lactobacillus acidophilus LA-5, Lactobacillus casei subsp. casei LC-01 and Bifidobacterium animalis subsp. lactis BB-12 on the composition and metabolic activities of the intestinal microbiota in human subjects during antibiotic treatment has been analyzed. Subjects that were placed on antibiotic therapy (amoxiciline/clavulanic or levofloxacin) were randomly assigned to receive a multispecies probiotic fermented milk (n = 122) or a placebo fermented milk (n = 125). Stool samples were collected at different intervals during the intervention study and analysed for faecal microbiota enumeration, probiotic species detection, enzyme activities and short chain fatty acid (SCFA) concentrations. The most predominant microbiota groups were total anaerobes, bacteroides and total aerobes. Probiotic species in fecal samples were analyzed by real-time PCR and results indicated that prevalence of the probiotic species within the probiotic group was below 50 % during the intervention study. Enzyme activities and SCFA concentrations showed a large inter-individual variability over the time in the placebo and probiotic groups, except to b-galactosidase activity, which was the most abundant in both groups over the time. In conclusion, the combination of L. acidophilus LA-5, L. casei subsp. casei LC-01 and B. animalis subsp. lactis Bb-12 taken in daily doses of 10exp9¿10exp10 colony-forming units (CFU) does not significantly influence (P>0.05) either the fecal microbiota or its metabolic activity in elderly hospitalized patients taking amoxicillin/clavulanate or levofloxacin. In addition, this combination of probiotic strains did not reduce the rate of occurrence of diarrohea in these patients.
Identifiersdoi: 10.1007/s00217-012-1851-3
issn: 1438-2377
e-issn: 1438-2385
Appears in Collections:(CIAL) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.