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Título

Structural rearrangements detected in newly-formed hexaploid tritordeum after three sequential FISH experiments with repetitive DNA sequences

AutorCabo, Sandra; Carvalho, Ana; Martín, Antonio ; Lima Brito, José
Palabras claveStructural rearrangements
Simple sequence repeats
Fluorescence in situ hybridization
Tritordeum
Wheat
Multicolour FISH
Nondenaturing FISH
Fecha de publicaciónabr-2014
EditorIndian Academy of Sciences
Springer
CitaciónJournal of Genetics 93(1): 183-188 (2014)
ResumenMitotic chromosomes of tritordeum (HchHchAABB; 2n = 6x = 42; HT27) and their respective parents, Hordeum chilense L. (HchHch; 2n = 2x = 14; H7) and Triticum turgidum (AABB; 2n = 4x = 28; T81), were analysed after three sequential fluorescence in situ hybridization (FISH) experiments performed with six probes: the simple sequence repeats (SSRs) (AG)10 and (AAC)5; genomic DNA from H. chilense; 45S rDNA (pTa71); 5S rDNA (pTa794); and pSc119.2, to achieve a comparative analysis about the distribution of these sequences in the three plant materials. The SSR (AG)10 probe showed eight hybridization signals on tritordeum chromosomes and six in the chromosomes of its male parent (durum wheat). No hybridization signals were detected in female parent H. chilense.
Versión del editorhttp://doi.org/10.1007/s12041-014-0328-5
URIhttp://hdl.handle.net/10261/101244
DOI10.1007/s12041-014-0328-5
Identificadoresdoi: 10.1007/s12041-014-0328-5
issn: 0022-1333
e-issn: 0973-7731
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