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Title

A Foodomics approach: CE-MS for comparative metabolomics of colon cancer cells treated with dietary polyphenols

AuthorsÇelebier, Mustafa; Ibáñez, Clara ; Simó, Carolina ; Cifuentes, Alejandro
KeywordsColon cancer
Metabolomics
Dietary polyphenols
CE
CE-MS
Foodomics
Issue Date2012
PublisherSpringer
CitationProtein Electrophoresis: 185-195 (2012)
SeriesMethods in Molecular Biology 869
AbstractThe potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area.
URIhttp://hdl.handle.net/10261/101222
DOI10.1007/978-1-61779-821-4_15
Identifiersdoi: 10.1007/978-1-61779-821-4_15
isbn: 978-1-61779-820-7
Appears in Collections:(CIAL) Libros y partes de libros
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