English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/101217
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Absorption of Casein antihypertensive peptides through an in vitro model of intestinal epithelium

AutorContreras, María del Mar; Recio, Isidra
Palabras claveAntihypertensive peptides
HPLC–MS
Caco-2 cells
HT29-MTX cells
Bioavailability
Fecha de publicación2012
EditorSpringer
CitaciónFood Digestion 3(1-3): 16-24 (2012)
ResumenA commercial casein hydrolysate, which contains peptides RYLGY and AYFYPEL as active molecules, has shown antihypertensive effects after acute and long-term administration. This study examines transepithelial absorption of RYLGY and AYFYPEL and derived digestion fragments using Caco-2, HT29-MTX -6 and co-culture 75 % Caco-2/25 % HT29-MTX-6 as predictive models and RP-HPLC-MS as analytical tool. Peptides RYLGY and AYFYPEL were absorbed intact through cell monolayers, although RYLGY was partly hydrolyzed by intestinal peptidases. Co-culture 75 % Caco-2/25 % HT29-MTX -6 exhibited intermediate properties, with regard to transepithelial electrical resistance, peptide hydrolysis, and absorption of the studied peptides, between Caco-2 and HT29-MTX-6 pure cultures. Interestingly, mucus layer that covered completely HT29-MTX-6 and co-culture monolayers was not a barrier for the absorption of the studied peptides. The apparent permeability values for absorptive transport across Caco-2 monolayers for RYLGY (0.22 × 10-6 cm/s) and AYFYPEL (0.26 × 10 -6 cm/s) were similar. These findings highlight that in vivo absorption of antihypertensive peptides RYLGY and AYFYPEL may occur partially as intact peptides. © 2012 Springer Science+Business Media, LLC.
URIhttp://hdl.handle.net/10261/101217
DOI10.1007/s13228-012-0020-2
Identificadoresdoi: 10.1007/s13228-012-0020-2
issn: 1869-1978
e-issn: 1869-1986
Aparece en las colecciones: (CIAL) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.