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A Role for RNAi in the Selective Correction of DNA Methylation Defects

AuthorsTeixeira, Felipe Karam; Heredia, Fabiana; Sarazin, Alexis; Roudier, François; Boccara, Martine; Ciaudo, Constance; Cruaud, Corinne; Poulain, Julie; Berdasco, María; Fernández Fraga, Mario; Voinnet, Olivier; Wincker, Patrick; Esteller, Manel; Colot, Vincent
KeywordsDNA methylation
Accidental changes
Epigenetics defects
Issue Date29-Jan-2009
PublisherAmerican Association for the Advancement of Science
CitationScience, doi: 10.1126/science.1165313 (in press)
AbstractDNA methylation is essential for silencing transposable elements and some genes in higher eukaryotes, implying that this modification must be tightly controlled. However, accidental changes in DNA methylation can be transmitted through mitosis, as in cancer, or meiosis, leading to epiallelic variation. Here, we demonstrate the existence of an efficient mechanism that protects against transgenerational loss of DNA methylation in Arabidopsis. Remethylation is specific to heavily methylated repeats that are targeted by the RNAi machinery. This process does not spread into flanking regions, is usually progressive over several generations, and faithfully restores wild-type methylation over target sequences, in an RNAi-dependent manner. Our findings suggest an important role for RNAi in protecting genomes against long-term epigenetic defects.
Description8 pages, 4 figures.-- PMID: 19179494 [PubMed].-- GEO accession number for small RNA sequences: GSE13419.
Supplementary information (Materials and Methods, Figs S1-S10, Tables S1-S4, Refs) available at: http://www.sciencemag.org/cgi/content/full/1165313/DC1
Publisher version (URL)http://dx.doi.org/10.1126/science.1165313
Appears in Collections:(CNB) Artículos
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