English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/101133
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Partial protease activity characterization of squid (Todaropsis eblanae) mantle

AutorAyensa, M. G; An, H.; Gómez Guillén, M. C. ; Montero García, Pilar ; Borderías, A. Javier
Palabras clavePota proteasas
Calamar
Inhibidores
Fecha de publicación1999
EditorSage Publications
CitaciónFood Science and Technology International 5: 391- 396 (1999)
ResumenProteolytic activity in mantle of Todaropsis eblanae was maximum at 40 and 65 °C. Several peaks of activity were detected over the pH range studied (1.5-9.5), indicating the presence of acidic, neutral and alkaline proteases, depending on the temperature. The substantial enzymic inhibition at acidic pH by the inhibitor trans-epoxysuccinyl-L-leucylamine-4-guanidine butane (E-64) revealed the pre-dominance of lysosomal cysteine proteases (cathepsins) which showed higher activity at 65 °C than at 40 °C. At 65 °C and pH 5.5 metallo-proteases were also detected by the inhibition with phenanthroline. Serine protease activity predominated at neutral pH (higher at 40 °C than at 65 °C), and cysteine proteases were detected at alkaline pH. There was evidence of cathepsin B and L activity at 65 °C and to a lesser degree at 40 °C. © 1999 Aspen Publishers, Inc.
URIhttp://hdl.handle.net/10261/101133
DOI10.1177/108201329900500504
Identificadoresdoi: 10.1177/108201329900500504
issn: 1082-0132
Aparece en las colecciones: (IF) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.