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dc.contributor.authorKarczmarek, Aneta-
dc.contributor.authorMartínez-Arteaga Baselga, Rocío-
dc.contributor.authorAlexeeva, Svetlana-
dc.contributor.authorHansen, Flemming G.-
dc.contributor.authorVicente, Miguel-
dc.contributor.authorNanninga, Nanne-
dc.contributor.authorBlaauwen, Tanneke den-
dc.identifier.citationMolecular Microbiology 2007, 65 (1): 51-63en_US
dc.descriptionIncluye 9 figuras y 1 tabla, más 2 figuras complementarias en archivo adjunto.en_US
dc.description.abstractThe bacterial actin homologue MreB forms a helix underneath the cytoplasmic membrane and was shown to be essential in the morphogenesis of the rod-shaped cells. Additionally, MreB was implicated to be involved in DNA segregation. However, in our hands the mreBCD deletion strain (PA340-678) grew without apparent DNA segregation defect, suggesting that the reported chromosome segregation inhibition could be caused by a temporarily effect of MreB inhibition or depletion. To assess the involvement of MreB in DNA segregation during the transition from rod to sphere, we compared the effect of A22 and the PBP2 inhibitor mecillinam on the percentage of cells with segregated nucleoids and the number of oriC foci in wild-type Escherichia coli cells. Cells became spherical in the same time window during both treatments and we could not detect any difference in the chromosome or oriC segregation between these two treatments. Additionally, flow cytometric analyses showed that A22 and mecillinam treatment gave essentially the same chromosome segregation pattern. We conclude that MreB is not directly involved in DNA segregation of E. coli.en_US
dc.description.sponsorshipThis work was supported by a European FP6 STREP Grant ‘COBRA’ LSHM-CT-2003-503335 (A. Karczmarek), a Molecule to Cell Grant 805.47.200 (S. Alexeeva)and a Vernieuwingsimpuls Grant 016.001.024 (T. den Blaauwen) both of the Netherlands Organization for Scientific Research (N.W.O.).en_US
dc.format.extent29696 bytes-
dc.publisherBlackwell Publishingen_US
dc.subjectEscherichia colien_US
dc.titleDNA and origin region segregation are not affected by the transition from rod to sphere after inhibition of Escherichia coli MreB by A22en_US
dc.description.peerreviewedPeer revieweden_US
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