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http://hdl.handle.net/10261/10069
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Sanglas, Laura | - |
dc.contributor.author | Avilés, Francesc X. | - |
dc.contributor.author | Huber, Robert | - |
dc.contributor.author | Gomis-Rüth, F. Xavier | - |
dc.contributor.author | Arolas, Joan L. | - |
dc.date.accessioned | 2009-01-29T07:51:06Z | - |
dc.date.available | 2009-01-29T07:51:06Z | - |
dc.date.issued | 2009-01-28 | - |
dc.identifier.citation | Proc. Natl. Acad. Sci. USA (PNAS) 106(6): 1743-1747 (2009) | en_US |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10261/10069 | - |
dc.description | 5 pages, 3 figures.-- Supplementary information (SI Materials and methods, Figs S1-S7, Tables S1-S2, 13 pages) available at: http://www.pnas.org/content/suppl/2009/01/28/0812623106.DCSupplemental/0812623106SI.pdf | - |
dc.description | Printed version published on Feb 10, 2009. | - |
dc.description.abstract | Roundworms of the genus Ascaris are common parasites of the human gastrointestinal tract. A battery of selective inhibitors protects them from host enzymes and the immune system. Here, a metallocarboxypeptidase (MCP) inhibitor, ACI, was identified in protein extracts from Ascaris by intensity-fading MALDI-TOF mass spectrometry. The 67-residue amino acid sequence of ACI showed no significant homology with any known protein. Heterologous overexpression and purification of ACI rendered a functional molecule with nanomolar equilibrium dissociation constants against MCPs, which denoted a preference for digestive and mast cell A/B-type MCPs. Western blotting and immunohistochemistry located ACI in the body wall, intestine, female reproductive tract, and fertilized eggs of Ascaris, in accordance with its target specificity. The crystal structure of the complex of ACI with human carboxypeptidase A1, one of its potential targets in vivo, revealed a protein with a fold consisting of two tandem homologous domains, each containing a β-ribbon and two disulfide bonds. These domains are connected by an α-helical segment and a fifth disulfide bond. Binding and inhibition are exerted by the C-terminal tail, which enters the funnel-like active-site cavity of the enzyme and approaches the catalytic zinc ion. The findings reported provide a basis for the biological function of ACI, which may be essential for parasitic survival during infection. | en_US |
dc.description.sponsorship | We acknowledge the help provided by European Molecular Biology Laboratory and European Synchrotron Radiation Facility synchrotron local contacts. This work was supported by the following grants: EU FP6 Strep Project LSHG-2006-018830 "CAMP"; EU FP7 Collaborative Project 223101 "AntiPathoGN"; BIO2007-68046, BIO2006-02668, and the CONSOLIDER INGENIO2010 Project "La Factoría de Cristalización" (CSD2006-00015) from Spanish public agencies. Funding was provided by European Synchrotron Radiation Facility for data collection. L.S. is a recipient of a predoctoral fellowship from the Ministry for Science and Innovation, Spain. J.L.A. is beneficiary of a "Juan de la Cierva" research contract awarded by the same ministry. | - |
dc.format.extent | 43048 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | National Academy of Sciences (U.S.) | en_US |
dc.rights | closedAccess | en_US |
dc.subject | Ascariasis | en_US |
dc.subject | Crystal structures | en_US |
dc.subject | Host resistance | en_US |
dc.subject | Immunolocalization | en_US |
dc.subject | Metallocarboxypeptidase inhibitor | en_US |
dc.title | Mammalian metallopeptidase inhibition at the defense barrier of Ascaris parasite | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1073/pnas.0812623106 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1073/pnas.0812623106 | en_US |
dc.identifier.pmid | 19179285 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
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