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Título: | A pRNA-induced structural rearrangement triggers 6S-1 RNA release from RNA polymerase in Bacillus subtilis |
Autor: | Beckmann, Benedict M.; Hoch, Philipp G.; Marz, Manja; Willkomm, Dagma K.; Salas, Margarita CSIC ORCID ; Hartmann, Robert K. | Fecha de publicación: | 14-feb-2012 | Editor: | Nature Publishing Group | Citación: | EMBO Journal 31: 1727- 1738 (2012) | Resumen: | Bacillus subtilis 6S-1 RNA binds to the housekeeping RNA polymerase (σ A-RNAP) and directs transcription of short 'product' RNAs (pRNAs). Here, we demonstrate that once newly synthesized pRNAs form a sufficiently stable duplex with 6S-1 RNA, a structural rearrangement is induced in cis, which involves base-pairing between sequences in the 5′-portion of the central bulge and nucleotides that become available as a result of pRNA invasion. The rearrangement decreases 6S-1 RNA affinity for σ A- RNAP. Among the pRNA length variants synthesized by σ A-RNAP (up to ∼14 nt), only the longer ones, such as 12-14-mers, form a duplex with 6S-1 RNA that is sufficiently long-lived to induce the rearrangement. Yet, an LNA (locked nucleic acid) 8-mer can induce the same rearrangement due to conferring increased duplex stability. We propose that an interplay of rate constants for polymerization (k(pol)), for pRNA:6S-1 RNA hybrid duplex dissociation (k(off)) and for the rearrangement (k(conf)) determines whether pRNAs dissociate or rearrange 6S-1 structure to trigger 6S-1 RNA release from σ A-RNAP. A bioinformatic screen suggests that essentially all bacterial 6S RNAs have the potential to undergo a pRNA-induced structural rearrangement. © 2012 European Molecular Biology Organization | All Rights Reserved. | URI: | http://hdl.handle.net/10261/100658 | DOI: | 10.1038/emboj.2012.23 | Identificadores: | issn: 0261-4189 |
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