English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/100442
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Characterization of the mbd cluster encoding the anaerobic 3-methylbenzoyl-CoA central pathway

AutorJuárez, Javier F. ; Zamarro, María Teresa ; Eberlein, C.; Boll, Matthias; Carmona, Manuel ; Díaz, Eduardo
Fecha de publicación4-jul-2012
EditorBlackwell Publishing
CitaciónEnvironmental Microbiology 15 (1) 148- 166 (2013)
ResumenThe mbd cluster encoding genes of the 3-methylbenzoyl-CoA pathway involved in the anaerobic catabolism of 3-methylbenzoate and m-xylene was characterized for the first time in the denitrifying β-Proteobacterium Azoarcus sp. CIB. The mbdA gene product was identified as a 3-methylbenzoate-CoA ligase required for 3-methylbenzoate activation; its substrate spectrum was unique in activating all three methylbenzoate isomers. An inducible 3-methylbenzoyl-CoA reductase (mbdONQP gene products), displaying significant amino acid sequence similarities to known class I benzoyl-CoA reductases catalysed the ATP-dependent reduction of 3-methylbenzoyl-CoA to a methyldienoyl-CoA. The mbdW gene encodes a methyldienoyl-CoA hydratase that hydrated the methyldienoyl-CoA to a methyl-6-hydroxymonoenoyl-CoA compound. The mbd cluster also contains the genes predicted to be involved in the subsequent steps of the 3-methylbenzoyl-CoA pathway as well as the electron donor system for the reductase activity. Whereas the catabolic mbd genes are organized in two divergent inducible operons, the putative mbdR regulatory gene was transcribed separately and showed constitutive expression. The efficient expression of the mbd genes required the oxygen-dependent AcpR activator, and it was subject of carbon catabolite repression by some organic acids and amino acids. Sequence analyses suggest that the mbd gene cluster was recruited by Azoarcus sp. CIB through horizontal gene transfer. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.
Descripción49 p.-3 tab.-7 fig.
Versión del editorhttp://dx.doi.org/10.1111/j.1462-2920.2012.02818.x
URIhttp://hdl.handle.net/10261/100442
DOI10.1111/j.1462-2920.2012.02818.x
ISSN1462-2912
E-ISSN1462-2920
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Juarez et al_2013_EnvironMicrobiol_15-1.pdf877,21 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.