English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10026
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides

AuthorsGhazi, Iraj ; Fernández Arrojo, Lucía ; Gómez de Segura, María Aránzazu; Alcalde Galeote, Miguel ; Plou Gasca, Francisco José ; Ballesteros Olmo, Antonio
Enzyme immobilization
Issue Date2006
PublisherAmerican Chemical Society
SeriesJournal of Agricultural and Food Chemistry 54(8): 2964-2968 (2006)
AbstractSugar syrup and molasses from beet processing containing 620 and 570 mg/ml sucrose, respectively, were assayed as low-cost and available substrates for the enzymatic synthesis of fructo-oligosaccharides (FOS). A commercial pectinase (Pectinex Ultra SP-L, from Aspergillus aculeatus) characterised by the presence of a transfructosylating activity, was used as biocatalyst. The FOS production increased when lowering the initial pH value of syrup (7.5) and molasses (8.9) to 5.5. Sugar syrup and molasses were diluted in order to reduce substrate viscosity; interestingly, the percentage of FOS with regards to total sugars remained almost constant, which indicated a high transferase to hydrolase ratio for this enzyme. Kinetics of FOS production was analysed. Using approx. 10 U transfructosylating activity per g sucrose, the FOS concentration reached a maximum of 388 mg/ml after 30 h using syrup and 235 mg/ml in 65 h with molasses. These values corresponded to approx. 56% and 49% (w/w), respectively, of the total amount of carbohydrates in the mixture. The enzyme was also covalently immobilised on an epoxy-activated polymethacrylate-based polymer (Sepabeads EC-EP5). We found that immobilised Pectinex Ultra SP-L can be efficiently applied to the synthesis of FOS using syrup and molasses as substrates.
Publisher version (URL)http://dx.doi.org/10.1021/jf053023b
Appears in Collections:(ICP) Artículos
Files in This Item:
File Description SizeFormat 
POSTPRINT-MOLASSES-FOS.pdf103,36 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.