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In aspergillus nidulans the suppressors suaA and suaC code for release factors eRF1 and eRF3 and suaD codes for a glutamine tRNA

AuthorsLiu, Wen; Mellado, Laura CSIC; Espeso, Eduardo A. CSIC ORCID ; Sealy-Lewis, Heather M.
KeywordsSuaC codes for eRF3
Mutations in A. nidulans
Suppression of nonsense
SuaA codes for eRF1
SuaD codes for a glutamine tRNA
Issue Date9-Apr-2014
PublisherGenetics Society of America
CitationG3 (Genes Genomes Genetics) 4: 1047- 1057 (2014)
AbstractIn Aspergillus nidulans, after extensive mutagenesis, a collection of mutants was obtained and four suppressor loci were identified genetically that could suppress mutations in putative chain termination mutations in different genes. Suppressor mutations in suaB and suaD have a similar restricted spectrum of suppression and suaB111 was previously shown to be an alteration in the anticodon of a gln tRNA. We have shown that like suaB, a suaD suppressor has a mutation in the anticodon of another gln tRNA allowing suppression of UAG mutations. Mutations in suaA and suaC had a broad spectrum of suppression. Four suaA mutations result in alterations in the coding region of the eukaryotic release factor, eRF1, and another suaA mutation has a mutation in the upstream region of eRF1 that prevents splicing of the first intron within the 59UTR. Epitope tagging of eRF1 in this mutant results in 20% of the level of eRF1 compared to the wildtype. Two mutations in suaC result in alterations in the eukaryotic release factor, eRF3. This is the first description in Aspergillus nidulans of an alteration in eRF3 leading to suppression of chain termination mutations. © 2014 Liu et al.
Description11 p.-2 tab.-8 fig.2 tab.S
Publisher version (URL)http://dx.doi.org/ 10.1534/g3.114.010702
Appears in Collections:(CIB) Artículos
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