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Title

Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus

AuthorsTarifa Álvarez, Gabriela; Villar del Paso, Ana; Pérez González, Clara; Yelamos Lorente, Ángeles; Marín Sánchez, Irene; Ceballos Miján, Nazaret; Peña López, Raúl; Jurado de Haro, Manuel; Muñoz Dorado, José; Pérez, Juana; Jiménez Zurdo, José I.
KeywordsPIIISA
Issue DateApr-2012
PublisherCSIC - Estación Experimental del Zaidín (EEZ)
CitationHigh School Students for Agricultural Science Research. Proceedings of the I Congress PIIISA: 1-8 (2012)
AbstractThe nitrogen‐fixing legume endosymbiont Sinorhizobium meliloti and the myxobacterium Myxococcus xanthus are both Gram‐negative bacteria which could potentially interact in soil. The objectives of this research work were to assess predation patterns of S. meliloti by M. xanthus and to set up an experimental system to study this process. We first characterized the growth phenotypes of several S. meliloti strains and a reference M. xanthus strain in different culture media. We observed that the best medium for S. meliloti was the rich TY medium, where bacteria exhibited markedly exopolysaccharide (EPS)‐ dependent mucous and non‐mucous phenotypes. In contrast, M. xanthus grew better in CTT medium where it showed a stable typical swarm phenotype. Co‐inoculation assays on CTT plates showed two patterns of S. meliloti responses to predation by myxobacteria, likely dependent on EPS production. We next tagged S. meliloti strains by conjugation with a plasmid‐borne kanamycin (Km) resistance gene. Predation assays in CTT broth revealed disappearance of S. meliloti tagged bacteria in the presence of M. xanthus as inferred from time‐course plate counts in Km‐supplemented TY medium. Our results provide a base‐line to investigate the molecular bases of bacterial predation in the future.
DescriptionProceedings of the I Congress PIIISA celebrado en la Estación Experimental del Zaidín, Granada, el 26 de abril de 2012.
URIhttp://hdl.handle.net/10261/100163
ISBN978‐84‐615‐8735‐3
84‐615‐8735‐9
ReferencesHigh School Students for Agricultural Science Research. Proceedings of the I Congress PIIISA. https://digital.csic.es/handle/10261/86940
Appears in Collections:(EEZ) Libros y partes de libros
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