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Title

Enzymatic degradation of Elephant grass (Pennisetum purpureum) stems: Influence of the pith and bark in the total hydrolysis

AuthorsPérez-Boada, Marta ; Prieto, Alicia ; Prinsen, Pepijn ; Forquin-Gomez, Marie-Pierre; Río Andrade, José Carlos del ; Gutiérrez Suárez, Ana ; Martínez, Ángel T. ; Faulds, Craig B.
KeywordsEnergy crop
Biomass utilization
Acetyl esterase
Glycoside hydrolases
Cosolvents
Issue Date24-Jun-2014
PublisherElsevier
CitationBioresource Technology 167 (2014) 469–475
AbstractThe internal pith of a high energy plant, Elephant grass (EG), was more extensively degraded (>50% dry matter) compared to the outer cortex (31%) or the whole stem (35%) by an enzyme preparation from Humicola insolens, Ultraflo. Reducing sugars and acetic acid release from the pith was also higher compared to the cortex. Supplementation of Ultraflo with a type-C feruloyl esterase increased the level of deacetylation but also led to reduced solubilisation. The addition of 20% dimethylsulfoxide (DMSO) as a co-solvent also reduced the solubility of EG by Ultraflo, although acetic acid release was increased, complimenting previous results found on model substrates. The presence of DMSO was also shown to have a protective effect on xylanase activity but not acetyl esterase activity in Ultraflo. Xylan in the biomass was preferentially solubilised by DMSO, while Ultraflo removed more glucose than xylose.
Description28 p.-1 tab.-4 fig.
Publisher version (URL)http://dx.doi.org/10.1016/j.biortech.2014.06.018
URIhttp://hdl.handle.net/10261/100162
DOI10.1016/j.biortech.2014.06.018
ISSN0960-8524
E-ISSN1873-2976
Appears in Collections:(CIB) Artículos
(IRNAS) Artículos
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