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Title

Identification of IgE-binding peptides in hen egg ovalbumin digested in vitro with human and simulated gastroduodenal fluids

AuthorsBenedé, Sara ; López-Expósito, Iván ; López-Fandiño, Rosina ; Molina, Elena
KeywordsEpitope
IgE
Human digestive fluids
In vitro digestion
Ovalbumin
Egg allergy
Issue Date2014
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 62(1): 152-158 (2014)
AbstractThe digestibility of the major egg allergen ovalbumin (OVA, Gal d 2) with human and simulated digestive fluids was assessed. Degradation of OVA was faster when treated with human fluids, particularly following duodenal digestion, leading to gastrointestinal digests with lower IgE binding. Gastric digestion with both systems yielded 52 identical cleavage sites and a similar peptide pattern with 47 peptides in common. Subsequent duodenal digestion showed that the human fluid released fewer and shorter peptides. Several high-frequency IgE-binding epitopes were detected among the fragments of molecular mass lower than 3 kDa identified in the digests: OVA (141-154) and OVA (164-176) in the gastrointestinal digests produced with human fluids; and OVA (125-134), OVA (159-172), OVA (141-154), OVA (188-198), OVA (326-336), and OVA (370-385) in the gastrointestinal digests produced with simulated fluids. The high binding frequency of the fragment OVA (370-385), which reacted with 80% of the sera from allergic patients used, was noteworthy. © 2013 American Chemical Society.
URIhttp://hdl.handle.net/10261/100047
DOI10.1021/jf404226w
Identifiersdoi: 10.1021/jf404226w
issn: 0021-8561
e-issn: 1520-5118
Appears in Collections:(CIAL) Artículos
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