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HPLC-ESI-QTOF-MS as a powerful analytical tool for characterising phenolic compounds in olive-leaf extracts

AuthorsQuirantes-Piné, Rosa; Lozano-Sánchez, Jesús; Herrero, Miguel ; Ibáñez, Elena ; Segura-Carretero, Antonio; Fernández-Gutiérrez, Alberto
KeywordsHigh-performance liquid chromatography
Phenolic compounds
Quadrupole time-of-flight
Olive leaves
Electrospray ionisation
Issue Date2013
PublisherJohn Wiley & Sons
CitationPhytochemical Analysis 24(3): 213-223 (2013)
Abstract[Introduction]: Olea europaea L. leaves may be considered a cheap, easily available natural source of phenolic compounds. In a previous study we evaluated the possibility of obtaining bioactive phenolic compounds from olive leaves by pressurised liquid extraction (PLE) for their use as natural anti-oxidants. The alimentary use of these kinds of extract makes comprehensive knowledge of their composition essential. [Objective]: To undertake a comprehensive characterisation of two olive-leaf extracts obtained by PLE using high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS). [Method]: Olive leaves were extracted by PLE using ethanol and water as extraction solvents at 150°C and 200°C respectively. Separation was carried out in a HPLC system equipped with a C 18-column working in a gradient elution programme coupled to ESI-QTOF-MS operating in negative ion mode. [Results]: This analytical platform was able to detect 48 compounds and tentatively identify 31 different phenolic compounds in these extracts, including secoiridoids, simple phenols, flavonoids, cinnamic-acid derivatives and benzoic acids. Lucidumoside C was also identified for the first time in olive leaves. [Conclusion]: The coupling of HPLC-ESI-QTOF-MS led to the in-depth characterisation of the olive-leaf extracts on the basis of mass accuracy, true isotopic pattern and tandem mass spectrometry (MS/MS) spectra. We may conclude therefore that this analytical tool is very valuable in the study of phenolic compounds in plant matrices. Copyright © 2012 John Wiley & Sons, Ltd.
Publisher version (URL)http://dx.doi.org/10.1002/pca.2401
Identifiersdoi: 10.1002/pca.2401
issn: 0958-0344
e-issn: 1099-1565
Appears in Collections:(CIAL) Artículos
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