DSpace Collection:
http://hdl.handle.net/10261/7103
2024-03-28T22:51:36Z
-
Detection and Quantification of Protein Aggregates in Plants
http://hdl.handle.net/10261/351844
Título: Detection and Quantification of Protein Aggregates in Plants
Autor: Phukan, Ujjal J.; Stael, Simon; Gonçalves, Amanda; Breusegem, Frank Van; Coll, Núria S.
Resumen: Protein quality control is an important aspect of stress recovery. It maintains protein homeostasis through a machinery of regulatory proteins such as chaperones and proteases. When the system recognizes accumulation of misfolded or aggregated proteins, the cell recruits a set of regulatory proteins to initiate protein quality control. To understand the dynamics of stress-mediated aggregate protein formation and recovery in plants, robust methods aimed at detecting and measuring such protein aggregates are needed. This will help us to deepen our understanding of protein quality control mechanisms in plants.
2024-03-26T08:39:59Z
-
Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity
http://hdl.handle.net/10261/351828
Título: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity
Autor: Iglesias-Sanchez, Ariadna; Navarro-Carcelen, Juan; Morelli, Luca; Rodriguez-Concepcion, Manuel
Resumen: Carotenoids are health-promoting plastidial isoprenoids with essential functions in plants as photoprotectants and photosynthetic pigments in chloroplasts. They also accumulate in specialized plastids named chromoplasts, providing color to non-photosynthetic tissues such as flower petals and ripe fruit. Carotenoid accumulation in chromoplasts requires specialized structures and proteins such as fibrillins (FBNs). The FBN family includes structural components of carotenoid sequestering structures in chromoplasts and members with metabolic roles in chloroplasts and other plastid types. However, the association of FBNs with carotenoids in plastids other than chromoplasts has remained unexplored. Here, we show that Arabidopsis (Arabidopsis thaliana) FBN6 interacts with phytoene synthase (PSY), the first enzyme of the carotenoid pathway. FBN6, but not FBN4 (a FBN that does not interact with PSY), enhances the activity of plant PSY (but not of the bacterial PSY crtB) in Escherichia coli cells. Overexpression of FBN6 in Nicotiana benthamiana leaves results in a higher production of phytoene, the product of PSY activity, whereas loss of FBN6 activity in Arabidopsis mutants dramatically reduces the production of carotenoids during seedling de-etiolation and after exposure to high light. Our work hence demonstrates that FBNs promote not only the accumulation of carotenoids in chromoplasts but also their biosynthesis in chloroplasts.
Descripción: © The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
2024-03-26T08:01:04Z
-
An LTR retrotransposon may activate the PsMYB10.2 gene expression and promote anthocyanin biosynthesis in the fruit flesh of Japanese plum – a success story of CRISPR-Cas9 enrichment sequencing strategy
http://hdl.handle.net/10261/351825
Título: An LTR retrotransposon may activate the PsMYB10.2 gene expression and promote anthocyanin biosynthesis in the fruit flesh of Japanese plum – a success story of CRISPR-Cas9 enrichment sequencing strategy
Autor: Fiol Garví, Arnau; Jurado-Ruiz, Federico; García, Sergio; Dujak, Christian; López Girona, Elena; Pacheco, Igor; Infante, Rodrigo; Aranzana, Maria José
Resumen: In this study, we review the methodology used to develop a molecular marker for fruit flesh color in Japanese plum, a species in which marker-assisted breeding is still in its infancy. We focus on the use and potential of the CRISPR-Cas9 enrichment sequencing, a novel methodology that attains the long-read target sequencing of genomic regions of interest. This technology allowed the sequencing of the highly polymorphic and duplicated LG3-MYB10 region of five plum cultivars in a cost-effective manner. This resulted in the identification of a structural variant associated with flesh color, which was an LTR retrotransposon of 2.8 kb inserted in the promoter of the PsMYB10.2 gene and allowed the design of an efficient molecular marker for the trait. The role of the PsMYB10.2 gene as an inducer of anthocyanin biosynthesis in the flesh was validated by transient overexpression in Japanese plum fruits. The long-terminal repeat retrotransposon might have a role in gene activation, as reported in other species. The study reviewed here is a great example that highlights the potential of CRISPR-Cas9 enrichment sequencing as a tool to identify genetic variations in genomic regions of interest, even if these are highly complex. As with Japanese plum and flesh color, this methodology could be a powerful tool for the development of molecular markers linked to traits of interest and agronomic value in any species.
Descripción: XXXI International Horticultural Congress (IHC2022): International Symposium on Breeding and Effective Use of Biotechnology and Molecular Tools in Horticultural Crops, 14-20 August 2022, Angers, France.
2024-03-26T07:41:33Z
-
Vegetation optical depth reveals changes in ecosystem-level water stress for global forests
http://hdl.handle.net/10261/350342
Título: Vegetation optical depth reveals changes in ecosystem-level water stress for global forests
Autor: Samuli Junttila; Adrià Descals; Iolanda Filella; Josep Peñuelas; Martin Brandt; Jean-Pierre Wigneron; Mikko Vastaranta
Resumen: Plant water stress due to climate change is posing a threat to various ecosystem services such as
carbon sequestration, food and wood production, and climate regulation. To address this issue,
methods are needed to assess and monitor plant water stress at various spatial and temporal
scales. Passive microwave emission observations from satellites have proven useful in monitoring
changes in vegetation water content and assessing plant water stress at a low spatial resolution (>
9 km). In this study, we used vegetation optical depth (VOD) and measurements of hydraulic
vulnerability to create a novel model for assessing ecosystem-level water stress. We used L-band
VOD and global measurements of xylem water potential at 88% loss of stem hydraulic conductivity
(P88) from the TRY database (including 1103 measurements of P88 from 463 species and nine
different vegetation biomes) to create a linear regression model between L-band VOD and biomelevel P88. We used monthly mean values of L-band VOD and calculated ratios of yearly minimum
and maximum VOD (L-VODmin/max) for each pixel to describe average variability in ecosystem-level
water content. The developed L-VODmin/max metric explained 75% of the variation in P88 at the
biome level (R
2
=0.75) indicating that the novel L-VODmin/max metric is capable of capturing changes
in plant water status. We then used the L-VODmin/max metric and daily climate data from the ERA5
to see if water stress has increased over time in the world's forests that are more water limited
(aridity index below 1.5). For these areas, we found a positive trend in maximum daily vapour
pressure deficit, which correlated negatively (p<0.05) with L-VODmin/max trend for the same time
period further confirming that L-VODmin/max is capable of explaining differences in plant water
status. Additionally, we examined the trend in L-VODmin/max for global forests for the same
2011-2020 period and found a significant negative trend (increasing water stress, p<0.05) for
forests in central Africa, southeast Asia, and eastern Australia, and a positive trend (decreasing
water stress) for boreal forests in North America and rainforests in Indonesia. Further studies are
required to confirm our results suggesting that some of the world's largest carbon sinks are
experiencing rapid changes in water stress as a result of climate change.
Descripción: Plant water stress due to climate change is posing a threat to various ecosystem services such as
carbon sequestration, food and wood production, and climate regulation. To address this issue,
methods are needed to assess and monitor plant water stress at various spatial and temporal
scales. Passive microwave emission observations from satellites have proven useful in monitoring
changes in vegetation water content and assessing plant water stress at a low spatial resolution (>
9 km). In this study, we used vegetation optical depth (VOD) and measurements of hydraulic
vulnerability to create a novel model for assessing ecosystem-level water stress. We used L-band
VOD and global measurements of xylem water potential at 88% loss of stem hydraulic conductivity
(P88) from the TRY database (including 1103 measurements of P88 from 463 species and nine
different vegetation biomes) to create a linear regression model between L-band VOD and biomelevel P88. We used monthly mean values of L-band VOD and calculated ratios of yearly minimum
and maximum VOD (L-VODmin/max) for each pixel to describe average variability in ecosystem-level
water content. The developed L-VODmin/max metric explained 75% of the variation in P88 at the
biome level (R
2
=0.75) indicating that the novel L-VODmin/max metric is capable of capturing changes
in plant water status. We then used the L-VODmin/max metric and daily climate data from the ERA5
to see if water stress has increased over time in the world's forests that are more water limited
(aridity index below 1.5). For these areas, we found a positive trend in maximum daily vapour
pressure deficit, which correlated negatively (p<0.05) with L-VODmin/max trend for the same time
period further confirming that L-VODmin/max is capable of explaining differences in plant water
status. Additionally, we examined the trend in L-VODmin/max for global forests for the same
2011-2020 period and found a significant negative trend (increasing water stress, p<0.05) for
forests in central Africa, southeast Asia, and eastern Australia, and a positive trend (decreasing
water stress) for boreal forests in North America and rainforests in Indonesia. Further studies are
required to confirm our results suggesting that some of the world's largest carbon sinks are
experiencing rapid changes in water stress as a result of climate change.
2024-03-13T15:28:15Z