DSpace Community:http://hdl.handle.net/10261/722024-03-28T11:36:40Z2024-03-28T11:36:40ZTrunk dielectric permittivity correlates with irrigation based on soil water content in fruit treesConesa, María R.Vera Muñoz, JuanConejero Puente, WenceslaoHernández Santana, V.Ruiz Sánchez, M. Carmenhttp://hdl.handle.net/10261/3506662024-03-18T13:37:25Z2024-03-18T08:16:03ZTítulo: Trunk dielectric permittivity correlates with irrigation based on soil water content in fruit trees
Autor: Conesa, María R.; Vera Muñoz, Juan; Conejero Puente, Wenceslao; Hernández Santana, V.; Ruiz Sánchez, M. Carmen
Resumen: Time-domain reflectometry (TDR) is an electromagnetic technique that measures the dielectric permittivity (K) which is a surrogate property influenced by water content. Advances in nanoelectronics have enabled the development of a TDR probe (TDR-305 N) to monitor changes in K, bulk electrical conductivity (ECbulk) and temperature (T) in a porous medium, such as a tree trunk. The main objective of this study was to assess the effectiveness of the TDR-305 N sensors for real-time monitoring changes in water content in the trunk of nectarine trees. Throughout the summer of 2022, irrigation was automatically managed with threshold values of soil water content (θv-soil) measured with capacitance probes. Different management allowed depletion (MAD) values were set to trigger irrigation: 50 % in July (moderate water deficit), 100 % in August (severe water deficit), and recovery to well-irrigated conditions in September. Discrete measurements of midday stem water potential (Ψs,md) and leaf gas exchange were made frequently. The results showed a progressive reduction of the measured physiological parameters, as well as of K and ECbulk and θv-soil decreased. Notably, Ψs,md reached a critically low value of -2.03 MPa, coinciding with pronounced and severe stomatal closure. Both K and Ψs,md, were able to explain the variations of θv-soil by more than 75 %. Daily, a positive relationship of K and ECbulk was observed, although ECbulk exhibited a stronger dependence on Ttrunk compared to K. Furthermore, K did not return to its initial values prior to the onset of water stress, possibly influenced by xylem cavitation and a reduction in leaf area during its senescence stage. The findings suggest that trunk permittivity measurements obtained using TDR-305 N sensors could be a reliable indicator for monitoring tree water status. However, further research is needed to determine the threshold values of trunk water content under non-limiting soil water conditions for accurate irrigation scheduling.
Descripción: 10 páginas.- 9 figuras.- 2 ilustraciones.- 1 tabla.- 62 referencias.-2024-03-18T08:16:03ZThe tomato calcium-permeable channel 4.1 (SlOSCA4.1) is a susceptibility factor for pepino mosaic virusRuiz-Ramón, FabiolaRodríguez Sepúlveda, PascualBretó, M. PauDonaire, LiviaHernando, YolandaAranda, Miguel A.http://hdl.handle.net/10261/3506232024-03-16T02:53:02Z2024-03-15T14:52:30ZTítulo: The tomato calcium-permeable channel 4.1 (SlOSCA4.1) is a susceptibility factor for pepino mosaic virus
Autor: Ruiz-Ramón, Fabiola; Rodríguez Sepúlveda, Pascual; Bretó, M. Pau; Donaire, Livia; Hernando, Yolanda; Aranda, Miguel A.
Resumen: The hyperosmolality-gated calcium permeable channel 4.1 (OSCA4.1) belongs to an evolutionarily conserved small family of mechano-sensitive channels. OSCA members may represent key players in plant resistance to drought and to pathogen infection but are scarcely studied. After screening for resistance to pepino mosaic virus (PepMV) a collection of 1000 mutagenized tomato families, we identified a mutant showing no symptoms and reduced virus accumulation. Resistance was mapped to chromosome 2 between positions 46 309 531 to 47 044 163, where a missense mutation caused the putative truncation of the OSCA4.1 protein. A CRISPR/Cas9 slosca4.1 mutant was resistant to PepMV, but not to tobacco mosaic virus or potato virus X. Inoculation of mutant and wild type tomato protoplasts showed that resistance was expressed in single cells, suggesting a role for SlOSCA4.1 in early viral function(s); congruently, SlOSCA4.1 re-localized to structures reminiscent of viral replication complexes. We propose that SlOSCA4.1 contributes to the correct regulation of the Ca2+ homeostasis necessary for optimal PepMV infection. PepMV is a pandemic virus that causes significant losses in tomato crops worldwide. In spite of its importance, no tomato-resistant varieties have been deployed yet; the mutant identified here has great potential to breed tomato varieties resistant to PepMV2024-03-15T14:52:30ZUnveiling metabotype clustering in resveratrol, daidzein, and ellagic acid metabolism: prevalence, associated gut microbiomes, and their distinctive microbial networks. APPENDIX A (Supplementary Material)Iglesias-Aguirre, Carlos E.Romo Vaquero, MaríaSelma, María VictoriaEspín de Gea, Juan Carloshttp://hdl.handle.net/10261/3505102024-03-16T02:52:04Z2024-03-15T06:51:50ZTítulo: Unveiling metabotype clustering in resveratrol, daidzein, and ellagic acid metabolism: prevalence, associated gut microbiomes, and their distinctive microbial networks. APPENDIX A (Supplementary Material)
Autor: Iglesias-Aguirre, Carlos E.; Romo Vaquero, María; Selma, María Victoria; Espín de Gea, Juan Carlos2024-03-15T06:51:50ZUnveiling metabotype clustering in resveratrol, daidzein, and ellagic acid metabolism: Prevalence, associated gut microbiomes, and their distinctive microbial networksIglesias-Aguirre, Carlos E.Romo Vaquero, MaríaSelma, María VictoriaEspín de Gea, Juan Carloshttp://hdl.handle.net/10261/3505092024-03-16T02:51:55Z2024-03-15T06:36:16ZTítulo: Unveiling metabotype clustering in resveratrol, daidzein, and ellagic acid metabolism: Prevalence, associated gut microbiomes, and their distinctive microbial networks
Autor: Iglesias-Aguirre, Carlos E.; Romo Vaquero, María; Selma, María Victoria; Espín de Gea, Juan Carlos
Resumen: The gut microbiota (GM) produces different polyphenol-derived metabolites, yielding high interindividual variability and hampering consistent health effects. GM metabotypes associated with ellagic acid (urolithin metabotypes A (UMA), B (UMB), and 0 (UM0)), resveratrol (lunularin -producers (LP) and non-producers (LNP)), and daidzein (equol-producers (EP) and non-producers (ENP)) are known. However, individual polyphenol-related metabotypes do not occur individually. In contrast, different combinations coexist (i.e., metabotype clusters, MCs). We report here for the first time these MCs, their distribution, and their associated GM in adult humans (n = 127) after consuming for 7 days a nutraceutical (pomegranate, Polygonum cuspidatum, and red clover extracts) containing ellagitannins + ellagic acid, resveratrol, and isoflavones. Urine metabolites (UHPLC-QTOF-MS) and fecal microbiota (16S rRNA sequencing) were analyzed. Ten MCs were identified: LP + UMB + ENP (22.7%), LP + UMA + ENP (21.3%), LP + UMA + EP (16.7%), LP + UMB + EP (16%), LNP + UMA + ENP (11.3%), LNP + UMB + ENP (5.3%), LNP + UMA + EP (3.3%), LNP + UMB + EP (2%), LNP + UM0 + EP (0.7%), and LNP + UM0 + ENP (0.7%). Sex, BMI, and age did not affect the distribution of metabotypes or MCs. Multivariate analysis (MaAslin2) revealed genera differentially present in individual metabotypes and MCs. Network analysis (MENA) showed the taxa acting as module hubs and connectors. Compositional and functional profiling, alpha and beta diversities, topological network features, and GM modulation by the nutraceutical differed depending on whether the entire cohort or each MC was considered. The nutraceutical did not change the composition of LP + UMA + EP (the most robust GM with the most associated functions) but increased its network connectors. This pioneering approach, joining GM’s compositional, functional, and network features in polyphenol metabolism, paves the way for identifying personalized GM-targeted strategies to improve polyphenol health benefits2024-03-15T06:36:16Z