DSpace Collection:http://hdl.handle.net/10261/12362024-03-19T03:15:49Z2024-03-19T03:15:49ZThermal tuning of enzyme activity by magnetic heatingArmenia, IlariaBussolari, FrancescaSánchez, ManuGallo-Cordova, ÁlvaroOvejero, Jesús G.Macedo de Melo, EduardoGamberoni, FedericaBorgese, MarinaSerio, SimoneGuisán, José ManuelFuente, Jesús M. de laPapait, RobertoGornati, RosalbaBernardini, GiovanniCassinelli, NicolasRother, DörteMorales, M. P.López-Gallego, FernandoGrazú, Valeriahttp://hdl.handle.net/10261/3505742024-03-16T02:52:37Z2024-03-15T11:21:37ZTítulo: Thermal tuning of enzyme activity by magnetic heating
Autor: Armenia, Ilaria; Bussolari, Francesca; Sánchez, Manu; Gallo-Cordova, Álvaro; Ovejero, Jesús G.; Macedo de Melo, Eduardo; Gamberoni, Federica; Borgese, Marina; Serio, Simone; Guisán, José Manuel; Fuente, Jesús M. de la; Papait, Roberto; Gornati, Rosalba; Bernardini, Giovanni; Cassinelli, Nicolas; Rother, Dörte; Morales, M. P.; López-Gallego, Fernando; Grazú, Valeria
Resumen: Enzymes are selective, specific, efficient, and more sustainable catalysts than their chemical counterparts. From an industrial perspective, multienzymatic systems to produce chemical compounds are particularly attractive since they comply with most green production principles including the minimization of wastes and their ecological footprints. However, the number of industrial processes based on the use of enzymes as catalysts remains limited. Indeed, incompatibility issues of enzymes with respect to reaction conditions or cross-reactivity are still challenging aspects that make one-pot enzyme cascade reactions hard to perform. The urgent need for more sustainable industrial processes has made different research fields, not accustomed to working together, join efforts to develop innovative technologies that could be key enabling to overcome these challenges. Among these technologies are those that seek to synergistically integrate the specific recognition and unique catalytic properties of enzymes with the size-dependent unique properties of nanoparticles. However, rather than giving a complete overview of the use of nanomaterials in biocatalysts, here it will be discussed the feasibility of triggering magnetic nanoparticles (MNPs) into hotspots by remote nanoactuation for the thermal modulation of discrete enzyme molecules activity with minimal collateral heating.; The possibility of heating discrete and defined local regions within a sample offers the potential to introduce a game-changing breakthrough in industrial biocatalysis by achieving coordinated enzyme function and thus concurrent multienzyme reactions. Therefore, not only the principles behind the activation of MNPs as hotspots, but also those examples that report the selective control of enzyme activity by magnetic heating, together with the foreseen challenges for the transfer of this technology to the industry, will be discussed.2024-03-15T11:21:37ZGTM-3, an extra-large pore enantioselective chiral zeolitic catalystSerna Valdés, Ramón de laNieto, DavidSainz, RaquelBernardo-Maestro, BeatrizMayoral, ÁlvaroPérez-Pariente, JoaquínGómez-Hortigüela Sainz, Luishttp://hdl.handle.net/10261/3099992023-05-31T01:37:51Z2023-05-30T08:55:39ZTítulo: GTM-3, an extra-large pore enantioselective chiral zeolitic catalyst
Autor: Serna Valdés, Ramón de la; Nieto, David; Sainz, Raquel; Bernardo-Maestro, Beatriz; Mayoral, Álvaro; Pérez-Pariente, Joaquín; Gómez-Hortigüela Sainz, Luis
Descripción: Trabajo presentado a la 20th International Zeolite Conference, celebrada en Valencia (España) del 3 al 8 de julio de 2022.2023-05-30T08:55:39ZWhite Paper 8: Clean safe and efficient energySerra Alfaro, José ManuelPérez-Coll, DomingoMíguez, HernánPalacín, M. RosaMartín-González, MarisolJordà, XavierRío Andrade, José Carlos delAbanades García, Juan CarlosConesa Cegarra, José CarlosChica Lara, AntonioRomero de Pablos, AnaDíaz Esteban, MarioCastro Martínez, ElenaBlanco Rodríguez, Clarahttp://hdl.handle.net/10261/2726092022-12-15T08:24:08Z2022-06-15T13:46:27ZTítulo: White Paper 8: Clean safe and efficient energy
Autor: Serra Alfaro, José Manuel; Pérez-Coll, Domingo; Míguez, Hernán; Palacín, M. Rosa; Martín-González, Marisol; Jordà, Xavier; Río Andrade, José Carlos del; Abanades García, Juan Carlos; Conesa Cegarra, José Carlos; Chica Lara, Antonio; Romero de Pablos, Ana; Díaz Esteban, Mario; Castro Martínez, Elena; Blanco Rodríguez, Clara
Resumen: The impact of energy production by conventional technologies on the environment and human health has promoted transition policies towards a new model for the energy sector. In this context, it is essential to identify the key challenges which favour the global implementation of a clean, safe and efficient energy system, focused on the ways in which energy is produced and stored, and the management of existing resources and their emissions.
Descripción: 255 p. : 17 cm2022-06-15T13:46:27ZImmobilization of enzymes on supports activated with glutaraldehyde: A very simple immobilization protocolLópez-Gallego, FernandoGuisán, José ManuelBetancor, Lorenahttp://hdl.handle.net/10261/2718342022-07-16T02:21:35Z2022-06-08T07:16:38ZTítulo: Immobilization of enzymes on supports activated with glutaraldehyde: A very simple immobilization protocol
Autor: López-Gallego, Fernando; Guisán, José Manuel; Betancor, Lorena
Resumen: In this chapter, we describe different approaches for the utilization of glutaraldehyde in protein immobilization. First, we focus on the covalent attachment of proteins to glutaraldehyde-activated matrixes. We describe conditions for the synthesis of such supports and provide an example of the immobilization and stabilization of a fructosyltransferase. We also describe how glutaraldehyde may be used for the cross-linking of protein–protein aggregates and protein adsorbed onto amino-activated matrixes. In these cases, glutaraldehyde bridges either two lysine groups from different protein molecules or a lysine from the protein structure and an amine group from the support. Examples of cross-linking are given for the immobilization of a D-amino acid oxidase on different amino-activated supports.2022-06-08T07:16:38Z