2024-03-28T10:04:11Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/899212016-02-18T02:47:38Zcom_10261_15com_10261_6col_10261_268
Application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria
Felske, A.
Vancanneyt, M.
Kersters, K.
Akkermans, A. D. L.
Strains belonging to the Gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (TGGE). This method allows the sequence-specific separation of amplified fragments of 16S rRNA genes. A total of 115 reference strains representing the majority of the species of the genera Aeromicrobium, Agromyces, Arthrobacter, Aureobacterium, Cellulomonas, Curtobacterium, Nocardioides and Terrabacter were characterized. Depending on the genus investigated, the resolution limit of the technique appeared to be at the species or genus level or intermediate between the two. Aberrant TGGE profiles of strains within particular taxa revealed genomic heterogeneity and generic misclassification of nine strains studied. Beyond that, indications of 16S rRNA gene heterogeneity were found within the genomes of three Curtobacterium strains. The misclassifications revealed by TGGE were confirmed using whole-cell fatty acid methyl ester analysis and subsequent comparison with a database. TGGE has been demonstrated to be a useful tool in bacterial taxonomy.
2014-01-24T15:20:17Z
2014-01-24T15:20:17Z
1999
2014-01-24T15:20:17Z
artÃculo
International Journal of Systematic Bacteriology 49: 113- 121 (1999)
http://hdl.handle.net/10261/89921
eng
openAccess
Society for General Microbiology