2024-03-28T08:47:44Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/897112021-06-30T07:49:21Zcom_10261_70com_10261_2col_10261_323
Integrated amperometric affinity biosensors using Co2+-tetradentane nitrilotriacetic acid modified disposable carbon electrodes. Application to the determination of beta-lactam antibiotics
Conzuelo, F.
Gamella, María
Campuzano, Susana
Martínez-Ruiz, Paloma
Esteban-Torres, María
Rivas, Blanca de las
Reviejo , A. J.
Muñoz, Rosario
Pingarrón, José M.
Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Ministerio de Educación, Cultura y Deporte (España)
A novel strategy for the construction of disposable amperometric affinity biosensors is described in this work. The approach uses a recombinant bacterial penicillin binding protein (PBP) tagged by an N-terminal hexahistidine tail which was immobilized onto Co2+−tetradentate nitrilotriacetic acid (NTA)-modified screen-printed carbon electrodes (SPCEs). The biosensor was employed for the specific detection and quantification of β-lactam antibiotics residues in milk, which was accomplished by means of a direct competitive assay using a tracer with horseradish peroxidase (HRP) for the enzymatic labeling. The amperometric response measured at −0.20 V versus the Ag pseudoreference electrode of the SPCE upon the addition of H2O2 in the presence of hydroquinone (HQ) as redox mediator was used as the transduction signal. The developed affinity sensor allowed limits of detection to be obtained in the low part-per-billion level for the antibiotics tested in untreated milk samples. Moreover, the biosensor exhibited a good selectivity against other antibiotics residues frequently detected in milk and dairy products. The analysis time was of approximately 30 min.
2014-01-21T15:41:50Z
2014-01-21T15:41:50Z
2013
2014-01-21T15:41:50Z
artículo
Analytical Chemistry 85(6): 3246-3254 (2013)
http://hdl.handle.net/10261/89711
10.1021/ac303604b
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100003176
http://dx.doi.org/10.13039/100012818
eng
Postprint
http://dx.doi.org/10.1021/ac303604b
Sí
S2009PPQ-164/AVANSENS
openAccess
American Chemical Society