2024-03-28T15:48:09Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/737312016-02-17T15:38:53Zcom_10261_75com_10261_6col_10261_328
Transcriptome sequencing identifies SPL7-regulated copper acquisition genes FRO4/FRO5 and the copper dependence of iron homeostasis in Arabidopsis
Bernal Ibáñez, María
Casero, David
Singha, Vasantika
Wilson, Grandon T.
Grande, Arne
Yang, Huijun
Dodani, Sheel C.
Huijser, Peter
Connolly, Erin L.
Merchant, Sabeeha S.
Krämer, Ute
24 Pags., 9 Figs., 2 Tabls., with Supplemental Data (15 Figs., 3 Tabls., 1 Method, 1 Data Set).
The transition metal copper (Cu) is essential for all living organisms but is toxic when present in excess. To identify Cu deficiency responses comprehensively, we conducted genome-wide sequencing-based transcript profiling of Arabidopsis thaliana wild-type plants and of a mutant defective in the gene encoding SQUAMOSA PROMOTER BINDING PROTEIN-LIKE7 (SPL7), which acts as a transcriptional regulator of Cu deficiency responses. In response to Cu deficiency, FERRIC REDUCTASE OXIDASE5 (FRO5) and FRO4 transcript levels increased strongly, in an SPL7-dependent manner. Biochemical assays and confocal imaging of a Cu-specific fluorophore showed that high-affinity root Cu uptake requires prior FRO5/FRO4-dependent Cu(II)-specific reduction to Cu(I) and SPL7 function. Plant iron (Fe) deficiency markers were activated in Cu-deficient media, in which reduced growth of the spl7 mutant was partially rescued by Fe supplementation. Cultivation in Cu-deficient media caused a defect in root-to-shoot Fe translocation, which was exacerbated in spl7 and associated with a lack of ferroxidase activity. This is consistent with a possible role for a multicopper oxidase in Arabidopsis Fe homeostasis, as previously described in yeast, humans, and green algae. These insights into root Cu uptake and the interaction between Cu and Fe homeostasis will advance plant nutrition, crop breeding, and biogeochemical research.
2013-04-05T12:25:03Z
2013-04-05T12:25:03Z
2012-02
artículo
Bernal M, Casero D, Singha V, Wilson GT, Grande A, Yang H, Dodani SC, Huijsere P, Connolly EL, Merchant SS, Krämer U. Transcriptome sequencing identifies SPL7-regulated copper acquisition genes FRO4/FRO5 and the copper dependence of iron homeostasis in Arabidopsis. Plant Cell 24 (2): 738-761 (2012)
1040-4651
http://hdl.handle.net/10261/73731
10.1105/tpc.111.090431
1532-298X
eng
http://dx.doi.org/10.1105/tpc.111.090431
openAccess
American Society of Plant Biologists