2024-03-29T15:46:06Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/727632020-10-27T10:47:16Zcom_10261_22com_10261_1com_10261_86col_10261_275col_10261_339
Ventricular enlargement associated with the panneural ablation of the podocalyxin gene
Nowakowski, Adam
Alonso-Martín, Sonia
González-Manchón, Consuelo
Larrucea, Susana
Fernández, Darío
Vilar-Egea, Maripaz
Cerdán, Sebastián
Sánchez Ayuso, Matilde
Parrilla, Roberto L.
Federación Española de Enfermedades Raras
Eusko Jaurlaritza
Instituto de Salud Carlos III
Ministerio de Ciencia e Innovación (España)
Podocalyxin (Podxl) is a type I membrane mucin-protein of the CD34 family abundantly expressed in kidney epithelial cells (podocytes) where it plays a crucial functional role. Podxl is also expressed in tissues other than kidney, like in brain, but its function is ignored. To investigate the functional role of podocalyxin (Podxl) in brain we produced the specific brain-ablation of the Podxl gen in mice by crossing Podxlfloxed/floxed mice, generated in our laboratory, to mice with pan-neural expression of recombinase Cre (Cre3). Podxl-/- mice show no apparent behavioral phenotype but their brains showed enlargement of ventricular volumes detected in vivo by MR imaging. The pattern of brain vasculature was of normal appearance but the thickness of the main carotid artery was significantly increased. Moreover, the histological analysis showed increased number of choroidal capillaries lining the ventricular spaces. These findings are analyzed in the light of the role likely played by podocalyxin in cell migration and cell-cell recognition during brain development and also on the consistent findings of increased ventricular spaces in human pathological disorders like schizophrenia. © 2009 Elsevier Inc. All rights reserved.
2013-03-22T12:22:55Z
2013-03-22T12:22:55Z
2010
2013-03-22T12:22:56Z
artículo
Molecular and Cellular Neurosciences 43(1):90-97(2010)
http://hdl.handle.net/10261/72763
10.1016/j.mcn.2009.09.011
http://dx.doi.org/10.13039/501100002924
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100003086
eng
Sí
closedAccess
Elsevier