2024-03-29T08:33:23Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/726132021-05-20T16:45:11Zcom_10261_86com_10261_1col_10261_339
Dedifferentiated adult articular chondrocytes: A population of human multipotent primitive cells
Fuente, Ricardo de la
Abad, José Luis
García-Castro, Javier
Fernández-Miguel, Gemma
Petriz, Jordi
Rubio, Daniel
Vicario-Abejón, Carlos
Guillén, Pedro
González, Manuel A.
Bernad, Antonio
Objective. To test the hypothesis that dedifferentiated adult human cartilage chondrocytes (HAC) are a true multipotent primitive population. Methods. Studies to characterize dedifferentiated HAC included cell cycle and quiescence analysis, cell fusion, flow-FISH telomere length assays, and ABC transporter analysis. Dedifferentiated HAC were characterized by flow cytometry, in parallel with bone marrow mesenchymal stem cells (MSC) and processed lipoaspirate (PLA) cells. The in vitro differentiation potential of dedifferentiated HAC was studied by cell culture under several inducing conditions, in multiclonal and clonal cell populations. Results. Long-term HAC cultures were chromosomically stable and maintained cell cycle dynamics while showing telomere shortening. The phenotype of dedifferentiated HAC was quite similar to that of human bone marrow MSC. In addition, this population expressed human embryonic stem cell markers. Multiclonal populations of dedifferentiated HAC differentiated to chondrogenic, osteogenic, adipogenic, myogenic, and neurogenic lineages. Following VEGF induction, dedifferentiated HAC expressed characteristics of endothelial cells, including AcLDL uptake. A total of 53 clonal populations of dedifferentiated HAC were efficiently expanded; 17 were able to differentiate to chondrogenic, osteogenic, and adipogenic lineages. No correlation was observed between telomere length or quiescent population and differentiation potential in the clones assayed. Conclusion. Dedifferentiated HAC should be considered a human multipotent primitive population. © 2004 Elsevier Inc. All rights reserved.
2013-03-21T11:17:08Z
2013-03-21T11:17:08Z
2004
2013-03-21T11:17:08Z
artículo
Experimental Cell Research 297(2):313-328(2004)
http://hdl.handle.net/10261/72613
10.1016/j.yexcr.2004.02.026
eng
closedAccess
Elsevier