2024-03-29T02:10:39Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/574832022-03-25T10:51:38Zcom_10261_47com_10261_8col_10261_300
The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species
Piñeiro, Carmen
Barros-Velázquez, J.
González Sotelo, Carmen
Gallardo, José Manuel
Two-dimensional electrophoresis
Isoelectric focusing
Flat fish
Protein
Identification
Differential characterization
7 páginas, 2 tablas, 4 figuras.-- The original publication is available at www.springerlink.com
The water-soluble proteins of nine flat fish
species of high commercial value, belonging to the
Pleuronectidae, Scophtalmidae, and Soleidae families,
were analyzed by two-dimensional (2D) electrophoresis,
this being carried out by nondenaturing isoelectric
focusing (IEF) in the 3.5–9.5 pH range and gradient
SDS-PAGE in the 12–14% range. Most of the major
proteins fell in the 3.5–6.9 pH range. From these, the
most specific proteins were in the acidic fraction
(pI~5.2; MW~16 kDa). Species-specific 2D protein
patterns were observed in all the nine species, and
more than 25 proteins could be individualized. The
combination of nondenaturing IEF and gradient SDSPAGE,
both in the absence of urea, allowed detailed
characterization of both the isoelectric points and molecular
weights of the major water-soluble proteins and
proved to be a valuable tool for the differential characterization
of the flat fish species studied.
2012-10-08T10:29:54Z
2012-10-08T10:29:54Z
1999
artículo
Zeitschrift für Lebensmitteluntersuchung und -Forschung A 208(5-6): 342-348 (1999)
1431-4630
http://hdl.handle.net/10261/57483
10.1007/s002170050427
eng
http://dx.doi.org/10.1007/s002170050427
openAccess
Springer