2024-03-28T13:22:15Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/553282014-04-16T07:39:08Zcom_10261_47com_10261_8col_10261_300
First detection of Pectenotoxin-11 and confirmation of OA-D8 diol-ester in Dinophysis acuta from European waters by LC-MS/MS
Pizarro, Gemita
Paz, Beatriz
Franco, José M.
Suzuki, Toshiyuki
Reguera, B.
Dinophysis acuta
DSP-toxins
Okadaic acid
Dinophysistoxins
OA-D8 diol-ester
Dinophysistoxin
Pectenotoxins
LC–MS/MS
8 páginas, 6 figuras
During November 2005, a dense bloom of Dinophysis spp. dominated (>97%) by Dinophysis
acuta in the Galician Rı´as Baixas (NW Spain), provided a unique opportunity to describe
the full toxin profile – including toxins that represent a low percentage and escape
detection in analyses of single-cell isolates – in plankton concentrates rich in this species.
Detection and identification of toxins were carried out by liquid chromatography coupled
to mass spectrometry (LC–MS/MS), a method that is based on their retention times (RT)
and the fragmentation patterns of their mass spectra. OA-D8 diol-ester and PTX11 were
detected in co-occurrence with okadaic acid analogues (OA, DTX2) and PTX2 in plankton
concentrates dominated by D. acuta. The presence of a PTX11-isomer, which was suggested
to be PTX13 or a novel PTX11-isomer, released in the sea water, was also confirmed in
samples obtained after deployment of passive samplers (SPATT) in situ at the time of the
D. acuta bloom maximum.
The amount of PTX11 per cell of D. acuta, estimated as PTX2 equivalents, ranged between
not detected and 2.2 pg cell 1, and represented a maximum of 2.9% of the total toxin
content. The variation in PTX11 content per cell of D. acuta, during a daily cycle, followed
the same pattern than that of PTX2, with maxima at 21:00 and 03:00 h (dark hours), but
the amounts per cell were one order of magnitude lower. This is the first report of PTX11,
together with the confirmation of OA-D8 diol-ester in D. acuta populations from Europe
2012-08-30T12:13:20Z
2012-08-30T12:13:20Z
2008
artículo
Toxicon 52(8): 889-896 (2008)
0041-0101
http://hdl.handle.net/10261/55328
10.1016/j.toxicon.2008.09.001
eng
http://dx.doi.org/10.1016/j.toxicon.2008.09.001
closedAccess
Elsevier