2024-03-29T15:12:06Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/517882018-09-12T11:14:01Zcom_10261_31891com_10261_2col_10261_31892
New possibilities for the valorization of olive oil by-products
Herrero, Miguel
Plaza, Merichel
Ibáñez, Elena
Ministerio de Educación y Ciencia (España)
Ministerio de Ciencia e Innovación (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
Asociación Hispano-Turca
El pdf del artículo es la versión pre-print.-- et al.
In this contribution, the capabilities of pressurized liquid extraction (PLE) using food-grade solvents, such as water and ethanol, to obtain antioxidant extracts rich on polyphenolic compounds from olive leaves are studied. Different extraction conditions were tested, and the PLE obtained extracts were characterized in vitro according to their antioxidant capacity (using the DPPH radical scavenging and the TEAC assays) and total phenols amounts. The most active extracts were obtained with hot pressurized water at 200°C (EC50 18.6μg/mL) and liquid ethanol at 150°C (EC50 27.4μg/mL), attaining at these conditions high extraction yields, around 40 and 30%, respectively. The particular phenolic composition of the obtained extracts was characterized by LC-ESI-MS. Using this method, 25 different phenolic compounds could be tentatively identified, including phenolic acids, secoiridoids, hydroxycinnamic acid derivatives, flavonols and flavones. Among them, hydroxytyrosol, oleuropein and luteolin-glucoside were the main phenolic antioxidants and were quantified on the extracts together with other minor constituents, by means of a UPLC-MS/MS method. Results showed that using water as extracting agent, the amount of phenolic compounds increased with the extraction temperature, being hydroxytyrosol the main phenolic component on the water PLE olive leaves extracts, reaching up to 8.542mg/g dried extract. On the other hand, oleuropein was the main component on the extracts obtained with ethanol (6.156-2.819mg/g extract). Results described in this work demonstrate the good possibilities of using PLE as a useful technique for the valorization of by-products from the olive oil industry, such as olive leaves. © 2011 Elsevier B.V.
2012-06-18T13:13:51Z
2012-06-18T13:13:51Z
2011
2012-06-18T13:13:51Z
artículo
Journal of Chromatography A 1218(42): 7511-7520 (2011)
http://hdl.handle.net/10261/51788
10.1016/j.chroma.2011.04.053
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003339
eng
http://dx.doi.org/10.1016/j.chroma.2011.04.053
openAccess
Elsevier