2024-03-28T20:30:03Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/514252021-11-22T13:12:32Zcom_10261_44755com_10261_1col_10261_44757
RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II
Zaratiegui, Mikel
Castro Álvarez, Elisa de
Marín, Laura
Antequera, Francisco
Ministerio de Ciencia e Innovación (España)
Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification during the DNA replication phase of the cell cycle. Here we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication in Schizosaccharomyces pombe. Co-transcriptional RNAi releases RNA polymerase II (Pol II), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes that spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification. © 2011 Macmillan Publishers Limited. All rights reserved.
2012-06-12T13:44:50Z
2012-06-12T13:44:50Z
2011
2012-06-12T13:44:50Z
artículo
Nature 479: 135-138 (2011)
http://hdl.handle.net/10261/51425
10.1038/nature10501
http://dx.doi.org/10.13039/501100004837
22002604
eng
closedAccess
Nature Publishing Group