2024-03-29T09:13:07Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/485362016-08-12T08:00:31Zcom_10261_103com_10261_1col_10261_356
Population differentiation and selective constraints in Pelargonium line pattern virus
Castaño, Aurora
Ruiz, Leticia
Elena, Santiago F.
Hernández Fort, Carmen
Plant RNA Virus
Chlorotic-ringspot virus
Cucumber Mosaic Virus
Endoplasmic-reticulum membrane
The genomic structure of Pelargonium line pattern virus (PLPV), a tentative member of a proposed new genus within the family Tombusviridae, has been recently determined. However, little is known about the genetic variability and population structure of this pathogen. Here, we have investigated the heterogeneity of PLPV isolates from different origins by sequence analysis of a 1817 nt fragment encompassing the movement (p7 and p9.7) and coat protein genes as well as flanking segments including the complete 3´untranslated region. We have evaluated the selective pressures operating on both viral proteins and RNA genome in order to asses the relative functional and/or structural relevance of different amino acid or nucleotide sites. The results of the study have revealed that distinct protein domains are under different selective constraints and that maintenance of certain primary and/or secondary structures in RNA regulatory sequences might be an important factor limiting viral heterogeneity. We have also performed covariation analyses to uncover potential dependencies among amino acid sites of the same protein or of different proteins. The detection of linked amino acid substitutions has permitted to draw a putative network of intra- and interprotein interactions that are likely required to accomplish the different steps of the infection cycle. Finally, we have obtained phylogenetic trees that support geographical segregation of PLPV sequences
2012-04-19T10:06:11Z
2012-04-19T10:06:11Z
2011-01
artículo
Virus Research 155/1:274-282 (2011)
http://hdl.handle.net/10261/48536
10.1016/j.virusres.2010.10.022
0168-1702
eng
openAccess
Elsevier