2024-03-28T11:31:19Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/337102022-07-16T01:01:08Zcom_10261_80com_10261_1col_10261_333
Molecular basis of FIR-mediated c-myc transcriptional control
Cukier, Cyprian D.
Hollingworth, David
Martin, Stephen
Kelly, Geoff
Díaz-Moreno, Irene
Ramos, Andrés
Far Upstream element
FUSE
FIR
c-Myc
FBP
19 páginas, 7 figuras, 1 tabla
The far upstream element (FUSE) regulatory system promotes a peak in the concentration of c-Myc during cell cycle. First, the FBP transcriptional activator binds to the FUSE DNA element upstream of the c-myc promoter. Then, FBP recruits its specific repressor (FIR), which acts as an on/off transcriptional switch. Here we describe the molecular basis of FIR recruitment, showing that the tandem RNA recognition motifs of FIR provide a platform for independent FUSE DNA and FBP protein binding and explaining the structural basis of the reversibility of the FBP-FIR interaction. We also show that the physical coupling between FBP and FIR is modulated by a flexible linker positioned sequentially to the recruiting element. Our data explain how the FUSE system precisely regulates c-myc transcription and suggest that a small change in FBP-FIR affinity leads to a substantial effect on c-Myc concentration.
2011-03-23T10:52:46Z
2011-03-23T10:52:46Z
2010-08-15
artículo
Nature Structural and Molecular Biology 17(9): 1058-1064 (2010)
1545-9993
PMC2964917
UKMS31552
http://hdl.handle.net/10261/33710
10.1038/nsmb.1883
20711187
eng
http://dx.doi.org/10.1038/nsmb.1883
openAccess
Nature Publishing Group