2024-03-28T13:37:54Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/324462022-07-18T09:41:22Zcom_10261_59com_10261_6col_10261_942
Advances in multilabeling for TEM and CLSM using primary antibodies derived from the same species. Application of Fab fragments to co-localize olive pollen allergens
Morales, Sonia
Rodríguez García, María I.
Alché Ramírez, Juan de Dios
Allergens
Antibodies
Fab fragments
immunofluorescence
immunogold
olive
pollen
multilabeling
Pages of the mentioned chapter: 607-613 (both included)
Publisher: Formatex Research Center
The cell distribution of multiple antigens can be simultaneously revealed by fluorescence and confocal laser scanning
microscopy (FM, CLSM) after using antibodies (Abs) conjugated to fluorophores with differential spectral characteristics.
The same multiple localization can be carried out at TEM (Transmission Electron Microscopy) by using gold particles of
different sizes. However, multilabeling studies often need to use primary Abs derived from the same host species. In these
cases, interference between different rounds of Abs must be prevented. Methods available to tackle this need are reviewed,
focusing particularly on the use of Fab fragments. Protocols regarding multiple localization of olive pollen targets (Ole e 1,
Ole e 2, Ole e 9 and actin) by CLSM and TEM are provided. They relay on the use of primary Abs raised in the same host
species (rabbit) and the application of Fab fragments.
2011-02-16T13:43:06Z
2011-02-16T13:43:06Z
2010-12
capítulo de libro
Microscopy: Science, Technology, Applications and Education
Volume 1 ISBN (13): 978-84-614-6189-9
http://hdl.handle.net/10261/32446
eng
openAccess
Formatex