2024-03-29T14:46:59Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2695112022-05-12T13:31:18Zcom_10261_7102com_10261_1col_10261_269508
Data from: Dynamic antagonism between phytochromes and PIF-family bHLHs induces selective reciprocal responses to light and shade in a rapidly responsive transcriptional network in Arabidopsis
Leivar, Pablo
Tepperman, James
Cohn, Megan M.
Monte, Elena
Al-Sady, Bassem
Erickson, Erika
Quail, Peter H.
Arabidopsis thaliana
Shade
Deetiolation
PIF
Phytochrome
Plants respond to shade-modulated light-signals, via the phytochrome (phy) system, by adaptive changes, collectively termed the shade avoidance syndrome (SAS). To examine the roles of the Phy-Interacting bHLH Factors, PIF1, 3, 4 and 5, in relaying this information to the transcriptional network, we compared the genome-wide expression profiles of wild-type and quadruple pif (pifq) mutants in response to shade. The data identify a subset of genes, enriched in transcription-factor-encoding loci, that respond rapidly (within 1 h), in a PIF-dependent manner, to the shade signal, and that contain promoter-located G-box-sequence motifs (CACGTG), known to be preferred PIF binding sites. These genes are thus potential direct targets of phy-PIF signaling that function in the primary transcriptional circuitry controlling downstream response-elaboration. A second subset of PIF-dependent, early-response genes, lacking G-box motifs, are enriched for auxin-responsive loci, suggestive of being indirect targets of phy-PIF signaling involved in the rapid cell-expansion known to be induced by shade. A meta-analysis comparing deetiolation- and shade-responsive transcriptomes identifies a further subset of G-box-containing genes that reciprocally display rapid repression and induction in response to light and shade signals at the inception of deetiolation and shade-avoidance, respectively. Collectively, these data define a core set of transcriptional and hormonal (auxin, cytokinin) processes that appear to be dynamically poised to react rapidly to changes in the light environment via perturbations in the mutually antagonistic actions of the phys and PIFs. Data from comparative analysis of the quadruple pifq and all triple pif-mutant combinations in response to light and shade, confirm that the PIF-quartet members act with overlapping redundancy on seedling morphogenesis and transcriptional regulation, but that the individual PIFs contribute differentially to these responses.
2022-05-12T13:07:17Z
2022-05-12T13:07:17Z
2012-06-08
dataset
Leivar, Pablo; Tepperman, James; Cohn, Megan M.; Monte, Elena; Al-Sady, Bassem; Erickson, Erika; Quail, Peter H.; 2012; Data from: Dynamic antagonism between phytochromes and PIF-family bHLHs induces selective reciprocal responses to light and shade in a rapidly responsive transcriptional network in Arabidopsis; Dryad; https://doi.org/10.5061/dryad.gd031k26
http://hdl.handle.net/10261/269511
10.5061/dryad.gd031k26
eng
Publisher's version
Leivar, Pablo; Tepperman, James; Cohn, Megan M.; Monte, Elena; Al-Sady, Bassem; Erickson, Erika; Quail, Peter H. Dynamic antagonism between phytochromes and PIF family basic helix-loop-helix factors induces selective reciprocal responses to light and shade in a rapidly responsive transcriptional network in Arabidopsis. Plant Cell 24(4): 1398-1419 (2012). https://doi.org/10.1105/tpc.112.095711 . http://hdl.handle.net/10261/248535
https://doi.org/10.5061/dryad.gd031k26
Sí
https://creativecommons.org/publicdomain/zero/1.0/
openAccess
Dryad