2024-03-29T12:49:19Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1994512022-03-10T05:31:01Zcom_10261_88com_10261_8col_10261_341
Taxonomy and functional interactions in upper and bottom waters of an oligotrophic high-mountain deep lake (Redon, Pyrenees) unveiled by microbial metagenomics
Llorens-Marès, Tomàs
Catalán, Jordi
Casamayor, Emilio O.
Bacteria
Functional genes
Alpine lakes
Biogeochemistry
Microbial biodiversity
High mountain lakes are, in general, highly sensitive systems to external forcing and
good sentinels of global environmental changes. For a better understanding of internal
lake processes, we examined microbial biodiversity and potential biogeochemical
interactions in the oligotrophic deep high-mountain Lake Redon (Pyrenees, 2240m
altitude) using shotgun metagenomics. We analyzed the two ends of the range of
environmental conditions found in Lake Redon, at 2 and 60 m depths. Bacteria were
the most abundant component of the metagenomic reads (> 90%) and the diversity
indices of both taxonomic (16S and 18S rRNA) and functional (carbon-, nitrogen-,
sulfur-, and phosphorous-cycling) related genes were higher in the bottom dark layer
than in the upper compartment. A marked segregation was observed both in
biodiversity and in the dominant energy and biomass generating pathways between
the extremes. The aerobic respiration was mainly dominated by heterotrophic
Burkholderiales at the top and Actinobacteria and Burkholderiales at the lake bottom.
The potential for an active nitrogen cycle (nitrogen fixation, nitrification, nitrite
oxidation, and nitrate reduction) was mainly found at 60 m, and potential for
methanogenesis, anaerobic ammonia oxidation and dissimilatory sulfur pathways
were only observed there. Some unexpected and mostly unseen energy and biomass
pathways were found relevant for the biogeochemical cycling in lake Redon, i.e.,
those related to carbon monoxide oxidation and phosphonates processing. We provide
a general scheme of the main biogeochemical processes that may operate in the
sentinel deep Lake Redon. This framework may help for a better understanding of the
whole lake metabolism.
2020-02-03T09:58:24Z
2020-02-03T09:58:24Z
2020
artículo
Science of the Total Environment : doi:10.1016/j.scitotenv.2019.135929 (2020)
0048-9697
http://hdl.handle.net/10261/199451
1879-1026
eng
Postprint
https://doi.org/10.1016/j.scitotenv.2019.135929
Sí
openAccess
Elsevier