2024-03-29T04:54:02Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1991172020-01-29T07:57:55Zcom_10261_5063com_10261_5col_10261_5066
Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains
Mortan, Siti Hatijah
Martín-González, Lucía
Vicent, T.
Caminal, Glòria
Nijenhuis, Ivonne
Adrian, Lorenz
Marco-Urrea, Ernest
Ministerio de Economía y Competitividad (España)
Caminal, Glòria [0000-0001-9646-6099]
1,1,2-Trichloroethane
Dehalococcoides
Dehalogenimonas
Fed-batch reactor
Organohalide-respiring bacteria
1,1,2-Trichloroethane (1,1,2-TCA) is a non-flammable organic solvent and common environmental contaminant in groundwater. Organohalide-respiring bacteria are key microorganisms to remediate 1,1,2-TCA because they can gain metabolic energy during its dechlorination under anaerobic conditions. However, all current isolates produce hazardous end products such as vinyl chloride, monochloroethane or 1,2-dichloroethane that accumulate in the medium. Here, we constructed a syntrophic co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains to achieve complete detoxification of 1,1,2-TCA to ethene. In this co-culture, Dehalogenimonas transformed 1,1,2-TCA via dihaloelimination to vinyl chloride, whereas Dehalococcoides reduced vinyl chloride via hydrogenolysis to ethene. Molasses, pyruvate, and lactate supported full dechlorination of 1,1,2-TCA in serum bottle co-cultures. Scale up of the cultivation to a 5-L bioreactor operating for 76 d in fed-batch mode was successful with pyruvate as substrate. This synthetic combination of bacteria with known complementary metabolic capabilities demonstrates the potential environmental relevance of microbial cooperation to detoxify 1,1,2-TCA. © 2017 Elsevier B.V.
2020-01-29T07:57:55Z
2020-01-29T07:57:55Z
2017-06-05
artículo
Journal of Hazardous Materials 331: 218-225 (2017)
http://hdl.handle.net/10261/199117
10.1016/j.jhazmat.2017.02.043
http://dx.doi.org/10.13039/501100003329
eng
Postprint
https://doi.org/10.1016/j.jhazmat.2017.02.043
Sí
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2013-48545-C2-1-R
openAccess
Elsevier