2024-03-28T16:53:14Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1862532021-02-03T13:45:02Zcom_10261_59com_10261_6com_10261_31891com_10261_2col_10261_312col_10261_31892
INFOGEST static in vitro simulation of gastrointestinal food digestion
Brodkorb, A.
Egger, Lotti
Alminger, Marie
Alvito, Paula
Assunção, Ricardo
Ballance, Simon
Bohn, Torsten
Bourlieu, C.
Boutrou, Rachel
Carrière, Frèderic
Clemente, Alfonso
Corredig, Milena
Dupont, Didier
Dufour, C.
Edwards, Cathrina
Golding, M.
Karakaya, S.
Kirkhus, Bente
Le Feunteun, S.
Lesmes, U.
Macierzanka, A.
Mackie, A. R.
Martins, Carla
Marze, Sébastien
McClements, David Julian
Ménard, Olivia
Minekus, M.
Portmann, Reto
Santos, Claudia N.
Souchon, Isabelle
Singh, R. Paul
Vegarud, Gerd E.
Wickham, Martin S. J.
Weitschies, Werner
Recio, Isidra
Centre National de la Recherche Scientifique (France)
Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.
2019-07-17T09:56:57Z
2019-07-17T09:56:57Z
2019
2019-07-17T09:56:57Z
artículo
Nature Protocols 14(4): 991-1014 (2019)
http://hdl.handle.net/10261/186253
10.1038/s41596-018-0119-1
http://dx.doi.org/10.13039/501100004794
30886367
eng
https://www.nature.com/articles/s41596-018-0119-1
Sí
closedAccess
Springer Nature