2024-03-28T22:05:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1648712018-05-18T00:57:03Zcom_10261_3284com_10261_1col_10261_3287
Plasmid conjugation-based logic gates
Cuevas, Ana
González-Gutiérrez, Sheila
Rodríguez-Patón, Alfonso
Garcillán-Barcia, M. Pilar
Cruz, Fernando de la
Resumen del póster presentado al XL Congreso de la Sociedad Española de Bioquímica y Biología Molecular (SEBBM), celebrado en Barcelona del 23 al 26 de octubre de 2017.
Conjugation is a natural communication mechanism among bacteria that relies in plasmids as vehicles. The conjugation hardware is highly specific and robust, allowing the independent transmission of different conjugative plasmids without interference. We constructed an E. coli derivative containing a chromosomally-inserted conjugative W-wire and engineered a recombinant plasmid series able to compute the basic logic gates YES, AND, OR. The computing strain was used as a donor in mating experiments and a reporter strain able to trigger the production of a fluorescent protein from the reporter plasmid was used as recipient. The functionality and specificity of these circuits constitute a proof of principle that cell-cell communication mediated by conjugative plasmids could be implemented
as a new tool in Synthetic Biology to distribute genetic circuits in bacterial populations.
2018-05-17T11:14:05Z
2018-05-17T11:14:05Z
2017
póster de congreso
XL SEBBM Congress (2017)
http://hdl.handle.net/10261/164871
eng
Sí
closedAccess